Construct: ORF TRCN0000473393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016082.1_s317c1
- Derived from:
- ccsbBroadEn_00862
- DNA Barcode:
- CCCAATTCTTTTCTGGGGTCTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL13RA2 (3598)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3598 | IL13RA2 | interleukin 13 receptor sub... | NM_000640.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttcgtttgc ttggctatcg gatgcttata tacctttctg ataagcacaa 121 catttggctg tacttcatct tcagacaccg agataaaagt taaccctcct caggattttg 181 agatagtgga tcccggatac ttaggttatc tctatttgca atggcaaccc ccactgtctc 241 tggatcattt taaggaatgc acagtggaat atgaactaaa ataccgaaac attggtagtg 301 aaacatggaa gaccatcatt actaagaatc tacattacaa agatgggttt gatcttaaca 361 agggcattga agcgaagata cacacgcttt taccatggca atgcacaaat ggatcagaag 421 ttcaaagttc ctgggcagaa actacttatt ggatatcacc acaaggaatt ccagaaacta 481 aagttcagga tatggattgc gtatattaca attggcaata tttactctgt tcttggaaac 541 ctggcatagg tgtacttctt gataccaatt acaacttgtt ttactggtat gagggcttgg 601 atcatgcatt acagtgtgtt gattacatca aggctgatgg acaaaatata ggatgcagat 661 ttccctattt ggaggcatca gactataaag atttctatat ttgtgttaat ggatcatcag 721 agaacaagcc tatcagatcc agttatttca cttttcagct tcaaaatata gttaaacctt 781 tgccgccagt ctatcttact tttactcggg agagttcatg tgaaattaag ctgaaatgga 841 gcataccttt gggacctatt ccagcaaggt gttttgatta tgaaattgag atcagagaag 901 atgatactac cttggtgact gctacagttg aaaatgaaac atacaccttg aaaaCAACAA 961 ATGAAACCCG ACAATTATGC TTTGTAGTAA GAAGCAAAGT GAATATTTAT TGCTCAGATG 1021 ACGGAATTTG GAGTGAGTGG AGTGATAAAC AATGCTGGGA AGGTGAAGAC CTATCGAAGA 1081 AAACTTTGCT ACGTTTCTGG CTACCATTTG GTTTCATCTT AATATTAGTT ATATTTGTAA 1141 CCGGTCTGCT TTTGCGTAAG CCAAACACCT ACCCAAAAAT GATTCCAGAA TTTTTCTGTG 1201 ATACATGCCC AACTTACTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCAATTCT TTTCTGGGGT CTAGGACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt