Construct: ORF TRCN0000473397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013549.1_s317c1
- Derived from:
- ccsbBroadEn_11904
- DNA Barcode:
- ACCCTAGTATATAAACATGTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OLA1 (29789)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473397
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29789 | OLA1 | Obg like ATPase 1 | NM_001328688.2 | 73% | 63.2% | 728_1025del;1125_1126insTAAAATT |
| 2 | human | 29789 | OLA1 | Obg like ATPase 1 | NM_013341.5 | 69.2% | 63% | 728_1088del;1188_1189insTAAAATT |
| 3 | human | 29789 | OLA1 | Obg like ATPase 1 | NM_001011708.3 | 29.5% | 23.5% | 0_1ins474;254_614del;714_715insTAAAATT |
| 4 | mouse | 67059 | Ola1 | Obg-like ATPase 1 | NM_030091.1 | 82.4% | 86.4% | (many diffs) |
| 5 | mouse | 67059 | Ola1 | Obg-like ATPase 1 | NM_025942.2 | 63.7% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 900
- ORF length:
- 834
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ccctaaaaag ggaggtgatg gaattaaacc acccccaatc attggaagat 121 ttggaacctc actgaaaatt ggtattgttg gattgccaaa tgttgggaaa tctactttct 181 tcaatgtgtt aaccaatagt caggcttcag cagaaaactt cccgttctgc actattgatc 241 ctaatgagag cagagtacct gtgccagatg aaaggtttga ctttctttgt caataccaca 301 aaccagcaag caaaattcct gcctttctaa atgtggtgga tattgctggc cttgtgaaag 361 gagctcacaa tgggcagggc ctggggaatg cttttttatc tcatattagt gcctgtgatg 421 gcatctttca tctaacacgt gcttttgaag atgatgatat cacgcacgtt GAAGGAAGTG 481 TAGATCCTAT TCGAGATATA GAAATAATAC ATGAAGAGCT TCAGCTTAAA GATGAGGAAA 541 TGATTGGGCC CATTATAGAT AAACTAGAAA AGGTGGCTGT GAGAGGAGGA GATAAAAAAC 601 TAAAACCTGA ATATGATATA ATGTGCAAAG TAAAATCCTG GGTTATAGAT CAAAAGAAAC 661 CTGTTCGCTT CTATCATGAT TGGAATGACA AAGAGATTGA AGTGTTGAAT AAACACTTAT 721 TTTTGACTTC AAAACCAATG GTCTACTTGG TTAATCTTTC TGAAAAAGAC TACATTAGAA 781 AGAAAAACAA ATGGCTGCTG GAAAGTACAG ACAACAAGGC AGAAATTATA TTGTTGAAGA 841 TGGAGATATT ATCTTCTTCA AATTTAACAC ACCTCAACAA CCGAAGAAGA AATAAAATTT 901 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 961 GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1021 TTTATATATC TTGTGGAAAG GACGAACCCT AGTATATAAA CATGTTCATA CGCGTTAAGT 1081 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt