Construct: ORF TRCN0000473408
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007486.2_s317c1
- Derived from:
- ccsbBroadEn_14765
- DNA Barcode:
- CAGAGCACTGGTTGCCGACCCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PI4KB (5298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473408
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001198773.3 | 99.9% | 99.8% | 786C>T;2396G>A |
| 2 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001198774.2 | 99.9% | 99.8% | 786C>T;2396G>A |
| 3 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001369624.1 | 99.9% | 99.8% | 786C>T;2396G>A |
| 4 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001369625.1 | 98.4% | 98.4% | 1_36del;822C>T;2432G>A |
| 5 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001330721.2 | 98% | 98% | 786C>T;910_954del;2441G>A |
| 6 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001369623.1 | 98% | 98% | 786C>T;910_954del;2441G>A |
| 7 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001369629.2 | 98% | 98% | 786C>T;910_954del;2441G>A |
| 8 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | XM_017001486.1 | 97.3% | 97.3% | 786C>T;2225_2287del;2459G>A |
| 9 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | XM_017001487.1 | 97.3% | 97.3% | 786C>T;2225_2287del;2459G>A |
| 10 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | XM_017001488.1 | 97.3% | 97.3% | 786C>T;2225_2287del;2459G>A |
| 11 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_002651.4 | 96.6% | 96.6% | (many diffs) |
| 12 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001369628.1 | 95.9% | 95.9% | (many diffs) |
| 13 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001369626.1 | 95.6% | 95.5% | (many diffs) |
| 14 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | XM_011509635.1 | 95.6% | 95.5% | (many diffs) |
| 15 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | XM_011509637.2 | 95.6% | 95.5% | (many diffs) |
| 16 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | XM_011509634.1 | 94.2% | 94.2% | (many diffs) |
| 17 | human | 5298 | PI4KB | phosphatidylinositol 4-kina... | NM_001198775.3 | 60.3% | 60.2% | 0_1ins951;1445G>A |
| 18 | mouse | 107650 | Pi4kb | phosphatidylinositol 4-kina... | NM_175356.3 | 92.5% | 98.3% | (many diffs) |
| 19 | mouse | 107650 | Pi4kb | phosphatidylinositol 4-kina... | NM_001293715.1 | 90.8% | 96.5% | (many diffs) |
| 20 | mouse | 107650 | Pi4kb | phosphatidylinositol 4-kina... | NM_001293716.1 | 55.9% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2469
- ORF length:
- 2403
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agatacagta gtggagcctg cccccttgaa gccaacttct gagcccactt 121 ctggcccacc agggaataat ggggggtccc tgctaagtgt catcacggag ggggtcgggg 181 aactatcagt gattgaccct gaggtggccc agaaggcctg ccaggaggtg ttggagaaag 241 tcaagctttt gcatggaggc gtggcagtct ctagcagagg caccccactg gagttggtca 301 atggggatgg tgtggacagt gagatccgtt gcctagatga tccacctgcc cagatcaggg 361 aggaggaaga tgagatgggg gccgctgtgg cctcaggcac agccaaagga gcaagaagac 421 ggcggcagaa caactcagct aaacagtctt ggctgctgag gctgtttgag tcaaaactgt 481 ttgacatctc catggccatt tcatacctgt ataactccaa ggagcctgga gtacaagcct 541 acattggcaa ccggctcttc tgctttcgca acgaggacgt ggacttctat ctgccccagt 601 tgcttaacat gtacatccac atggatgagg acgtgggtga tgccattaag ccctacatag 661 tccaccgttg ccgccagagc attaactttt ccctccagtg tgccctgttg cttggggcct 721 attcttcaga catgcacatt tccactcaac gacactcccg tgggaccaag ctacggaagc 781 tgatcctctc agatgagcta aagccagctc acaggaagag ggagctgccc tccttgagcc 841 cggcccctga tacagggctg tctccctcca aaaggactca ccagcgctct aagtcagatg 901 ccactgccag cataagtctc agcagcaacc tgaaacgaac agccagcaac cctaaagtgg 961 agaatgagga tgagcctgtt cgactggctc ctgagagaga attcatcaag tccctgatgg 1021 cgatcggcaa gcggctggcc acgctcccca ccaaagagca gaaaacacag aggctgatct 1081 cagagctctc cctgctcaac cataagctcc ctgcccgagt ctggctgccc actgctggct 1141 ttgaccacca cgtggtccgt gtaccccaca cacaggctgt tgtcctcaac tccaaggaca 1201 aggctcccta cctgatttat gtggaagtcc ttgaatgtga aaactttgac accaccagtg 1261 tccctgcccg gatccccgag aaccgaattc ggagtacgag gtccgtagaa aacttgcccg 1321 aatgtggtat tacccatgag cagcgagctg gcagcttcag cactgtgccc aactatgaca 1381 acgatgatga ggcctggtcg gtggatgaca taggcgagct gcaagtggag ctccccgaag 1441 tgcataccaa cagctgtgac aacatctccc agttctctgt ggacagcatc accagccagg 1501 agagcaagga gcctgtgttc attgcagcag gggacatccg ccggcgcctt tcggaacagc 1561 tggctcatac cccgacagcc ttcaaacgag acccagaaga tccttctgca gttgctctca 1621 aagagccctg gcaggagaaa gtacggcgga tcagagaggg ctccccctac ggccatctcc 1681 ccaattggcg gctcctgtca gtcattgtca agtgtgggga tgaccttcgg caagagcttc 1741 tggcctttca ggtgttgaag caactgcagt ccatttggga acaggagcga gtgccccttt 1801 ggatcaagcc atacaagatt cttgtgattt cggctgatag tggcatgatt gaaccagtgg 1861 tcaatgctgt gtccatccat caggtgaaga aacagtcaca gctctccttg ctcgattact 1921 tcctacagga gcacggcagt tacaccactg aggcattcct cagtgcacag cgcaattttg 1981 tgcaaagttg tgctgggtac tgcttggtct gctacctgct gcaagtcaag gacagacaca 2041 atgggaatat ccttttggac gcagaaggcc acatcaTCCA CATCGACTTT GGCTTCATCC 2101 TCTCCAGCTC ACCCCGAAAT CTGGGCTTTG AGACGTCAGC CTTTAAGCTG ACCACAGAGT 2161 TTGTGGATGT GATGGGCGGC CTGGATGGCG ACATGTTCAA CTACTATAAG ATGCTGATGC 2221 TGCAAGGGCT GATTGCCGCT CGGAAACACA TGGACAAGGT GGTGCAGATC GTGGAGATCA 2281 TGCAGCAAGG TTCTCAGCTT CCTTGCTTCC ATGGCTCCAG CACCATTCGA AACCTCAAAG 2341 AGAGGTTCCA CATGAGCATG ACTGAGGAGC AGCTGCAGCT GCTGGTGGAG CAGATGGTGG 2401 ATGGCAGTAT GCGGTCTATC ACCACCAAAC TCTATGACGG CTTCCAGTAC CTCACCAACG 2461 ACATCATGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2521 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2581 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAGAGC ACTGGTTGCC GACCCGAGAC 2641 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt