Construct: ORF TRCN0000473410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014223.4_s317c1
- Derived from:
- ccsbBroadEn_14946
- DNA Barcode:
- AGTCAAGTTCTTCGCAAACAGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IP6K1 (9807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9807 | IP6K1 | inositol hexakisphosphate k... | NM_001242829.1 | 99.9% | 100% | 1309C>A |
| 2 | human | 9807 | IP6K1 | inositol hexakisphosphate k... | NM_153273.4 | 99.9% | 100% | 1309C>A |
| 3 | human | 9807 | IP6K1 | inositol hexakisphosphate k... | NM_001006115.2 | 62.5% | 62.5% | 0_1ins495;814C>A |
| 4 | mouse | 27399 | Ip6k1 | inositol hexaphosphate kina... | NM_013785.2 | 89.8% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1389
- ORF length:
- 1323
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgtttgtcaa accatggaag tggggcagta tggcaagaat gcaagtcggg 121 ctggagaccg gggagtcctc ctggagccct tcatccacca agtaggcgga cacagcagca 181 tgatgcgtta cgacgatcac actgtgtgca agcccctcat ctcccgggaa cagcgctttt 241 acgagtccct ccctcccgaa atgaaggagt tcacccctga atacaaaggc gtggtatctg 301 tctgttttga gggggacagt gatggttaca tcaacttagt ggcctatcct tatgtggaaa 361 gtgagactgt ggaacaggat gacacaacag aacgggagca acctcggcgc aaacactccc 421 gccggagcct gcaccggtca ggcagtggca gtgaccacaa ggaggagaaa gccagcctgt 481 cccttgagac ctctgagagc tcacaggagg caaagagtcc gaaggtggag ctgcacagcc 541 actcagaggt ccctttccag atgctagatg gcaacagtgg cttgagttct gagaagatca 601 gccacaaccc ctggagcctg cgttgtcaca agcagcagct gagccgcatg cgctccgagt 661 ccaaggaccg aaagctctac aagttcctcc tgcttgagaa cgtggtgcac cacttcaagt 721 acccctgcgt gttggacctg aagatgggca cgcggcagca tggcgatgac gcgtcagctg 781 agaaggcagc ccggcagatg cggaaatgcg agcagagcac atcagccacg ctgggcgtca 841 gggtctgcgg catgcaggtg taccagctgg acacagggca ttacctctgc aggaacaagt 901 actatggccg tgggctctcc attgaaggct tccgcaatgc cctctatcaa tatctgcaca 961 atggcctgga cctgcgacgt gacctgtttg agcctatcct gagcaaactg cggggccTGA 1021 AAGCTGTGCT GGAGCGGCAG GCCTCTTACC GCTTCTACTC CAGTTCCCTG CTTGTCATCT 1081 ATGATGGCAA GGAGTGCCGG GCTGAGTCCT GCCTGGACCG CCGGTCTGAG ATGCGTCTCA 1141 AGCACCTGGA CATGGTGCTC CCTGAGGTGG CGTCATCCTG TGGCCCCAGC ACCAGCCCCA 1201 GCAACACCAG CCCCGAGGCG GGTCCCTCCT CTCAGCCCAA GGTGGATGTC CGCATGATTG 1261 ACTTTGCACA CAGCACATTC AAGGGCTTCC GGGATGACCC CACCGTGCAT GATGGGCCAG 1321 ACAGAGGCTA CGTGTTTGGC CTGGAGAACC TCATCAGCAT CATGGAACAG ATGAGGGACG 1381 AGAACCAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1441 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1501 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTCAA GTTCTTCGCA AACAGGAGAC 1561 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt