Construct: ORF TRCN0000473422
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011768.1_s317c1
- Derived from:
- ccsbBroadEn_08372
- DNA Barcode:
- AGTACGAAAGAACTTCTCCCTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIAE (54414)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473422
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54414 | SIAE | sialic acid acetylesterase | NM_170601.5 | 99.8% | 100% | 225G>A;1515C>A |
| 2 | human | 54414 | SIAE | sialic acid acetylesterase | NM_001199922.1 | 93.1% | 93.3% | 0_1ins105;120G>A;1410C>A |
| 3 | human | 54414 | SIAE | sialic acid acetylesterase | XM_017017930.1 | 63.4% | 63.4% | 0_1ins573;942C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1635
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cgcgccgggg cttgtactcg ggctggtgct gccattaatc ctgtgggccg 121 acagaagtgc aggtattggt tttcgctttg cttcatacat caataatgat atggtgctgc 181 agaaggagcc tgctggggca gtgatatggg gcttcggtac acctggagcc acagtgaccg 241 tgaccctgcg ccaaggtcag gaaaccatca tgaagaaagt gaccagtgta aaagctcact 301 ctgatacgtg gatggtggta ctggatccta tgaagcctgg aggacctttc gaagtgatgg 361 cacaacagac tttggagaaa ataaacttca ccctgagagt tcatgacgtc ctgtttggag 421 atgtctggct ctgtagtggg cagagtaaca tgcagatgac tgtgttacag atatttaatg 481 ctacaaggga gttgtctaac actgcggcat atcagtctgt ccgcatcctc tctgtctctc 541 ccattcaagc agagcaggag ctggaggacc ttgttgcggt tgacttgcag tggtctaagc 601 ccacctcaga aaacttaggc catggatatt tcaagtacat gtcagcagtg tgctggctct 661 ttggacgtca cctttatgac actctgcagt atcccatcgg gctgatcgcc tccagctggg 721 gcgggacacc cattgaagcc tggtcatctg gacggtcact gaaagcctgt ggggtcccta 781 aacaagggtc cattccatac gattctgtaa ctggtcccag taagcactct gttctctgga 841 atgccatgat ccatccactg tgcaatatga ctctgaaagg ggtagtatgg taccaggggg 901 agtccaatat aaattataac acggatctgt acaattgcac attccctgca ctcatcgaag 961 actggcgtga aaccttccac cgtggttccc aggggcagac ggagcgtttc ttcccatttg 1021 gacttgtcca gttatcttca gatttgtcta agaagagctc agacgatgga tttccccaga 1081 tccgttggca tcaaacagca gacttcggct atgtccccaa cccaaagatg cccaatactt 1141 tcatggctgt agctatggat ctctgtgata gagactcgcc ttttGGCAGC ATCCACCCTC 1201 GAGATAAACA GACTGTGGCT TATCGGCTGC ATTTGGGGGC CCGTGCTCTG GCTTATGGTG 1261 AGAAGAATTT GACCTTTGAA GGACCACTGC CTGAGAAGAT AGAACTCTTG GCTCACAAGG 1321 GGCTGCTCAA TCTCACATAT TACCAGCAAA TCCAGGTGCA GAAAAAGGAC AACAAGATAT 1381 TTGAGATCTC CTGTTGCAGT GACCATCGAT GCAAGTGGCT TCCAGCTTCT ATGAACACCG 1441 TCTCCACCCA GTCCCTGACC CTGGCGATCG ATTCTTGTCA TGGCACTGTG GTTGCTCTCC 1501 GCTATGCTTG GACCACGTGG CCTTGTGAAT ATAAGCAGTG TCCCCTATAC CACCCCAGTA 1561 GTGCCCTGCC AGCCCCTCCA TTCATTGCTT TCATTACAGA CCAGGGTCCT GGACATCAGA 1621 GCAATGTTGC TAAATGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGTACGAAAG AACTTCTCCC 1801 TATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt