Construct: ORF TRCN0000473427
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005740.1_s317c1
- Derived from:
- ccsbBroadEn_01717
- DNA Barcode:
- AATACTGGTTAATAGTTTAAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSHR (7253)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473427
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7253 | TSHR | thyroid stimulating hormone... | NM_001018036.3 | 100% | 100% | |
| 2 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_005268039.1 | 100% | 100% | |
| 3 | human | 7253 | TSHR | thyroid stimulating hormone... | NM_001142626.3 | 92.3% | 92.3% | 693_755del |
| 4 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_006720245.1 | 92.3% | 92.3% | 693_755del |
| 5 | human | 7253 | TSHR | thyroid stimulating hormone... | NM_000369.3 | 32% | 30.1% | (many diffs) |
| 6 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_005268037.4 | 32% | 30.1% | (many diffs) |
| 7 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_011537119.2 | 19.6% | 16.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gccggcggac ttgctgcagc tggtgctgct gctcgacctg cccagggacc 121 tgggcggaat ggggtgttcg tctccaccct gcgagtgcca tcaggaggag gacttcagag 181 tcacctgcaa ggatattcaa cgcatcccca gcttaccgcc cagtacgcag actctgaagc 241 ttattgagac tcacctgaga actattccaa gtcatgcatt ttctaatctg cccaatattt 301 ccagaatcta cgtatctata gatgtgactc tgcagcagct ggaatcacac tccTTCTACA 361 ATTTGAGTAA AGTGACTCAC ATAGAAATTC GGAATACCAG GAACTTAACT TACATAGACC 421 CTGATGCCCT CAAAGAGCTC CCCCTCCTAA AGTTCCTTGG CATTTTCAAC ACTGGACTTA 481 AAATGTTCCC TGACCTGACC AAAGTTTATT CCACTGATAT ATTCTTTATA CTTGAAATTA 541 CAGACAACCC TTACATGACG TCAATCCCTG TGAATGCTTT TCAGGGACTA TGCAATGAAA 601 CCTTGACACT GAAGCTGTAC AACAATGGCT TTACTTCAGT CCAAGGATAT GCTTTCAATG 661 GGACAAAGCT GGATGCTGTT TACCTAAACA AGAATAAATA CCTGACAGTT ATTGACAAAG 721 ATGCATTTGG AGGAGTATAC AGTGGACCAA GCTTGCTGCT ACCTCTTGGA AGAAAGTCCT 781 TGTCCTTTGA GACTCAGAAG GCCCCACGCT CCAGTATGCC ATCATGCCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA AATACTGGTT AATAGTTTAA AGCGACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt