Construct: ORF TRCN0000473428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014248.1_s317c1
- Derived from:
- ccsbBroadEn_05968
- DNA Barcode:
- CGAGTATAACTGTGCTCAGCGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDA (978)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473428
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 978 | CDA | cytidine deaminase | NM_001785.3 | 99.5% | 99.3% | 79A>C;435C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccagaagcgt cctgcctgca ccctgaagcc tgagtgtgtc cagcagctgc 121 tggtttgctc ccaggaggcc aagcagtcag cctactgccc ctacagtcac tttcctgtgg 181 gggctgccct gctcacccag gaggggagaa tcttcaaagg gtgcaacata gaaaatgcct 241 gctacccgct gggcatctgt gctgaacgga ccgctatcca gaaggccgtc tcagaagggt 301 acaaggattt cagggcaatt gctatcgcca gtgacatgca agatgatttt atctcTCCAT 361 GTGGGGCCTG CAGGCAAGTC ATGAGAGAGT TTGGCACCAA CTGGCCCGTG TACATGACCA 421 AGCCGGATGG TACGTATATT GTCATGACGG TCCAGGAGCT GCTGCCCTCC TCCTTTGGGC 481 CTGAGGACCT GCAGAAGACT CAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GAGTATAACT 661 GTGCTCAGCG TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att