Construct: ORF TRCN0000473430

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008960.1_s317c1
Derived from:
ccsbBroadEn_07625
DNA Barcode:
AGACATCTAGTTTTCCTGGCCAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEMA3C (10512)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473430
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10512 SEMA3C semaphorin 3C NM_006379.5 99.8% 100% (many diffs)
2 human 10512 SEMA3C semaphorin 3C NM_001350120.2 97.4% 97.6% (many diffs)
3 human 10512 SEMA3C semaphorin 3C NM_001350121.1 92% 92.2% (many diffs)
4 mouse 20348 Sema3c sema domain, immunoglobulin... NM_013657.5 88.2% 97% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2319
ORF length:
2253
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc attccggaca atttgcgtgt tggttggagt atttatttgt tctatctgtg
121tgaaaggatc ttcccagccc caagcaagag tttatttaac atttgatgaa cttcgagaaa
181ccaagacctc tgaatacttc agcctttccc accatccttt agactacagg attttattaa
241tggatgaaga tcaggaccgg atatatgtgg gaagcaaaga tcacattctt tccctgaata
301ttaacaatat aagtcaagaa gctttgagtg ttttctggcc agcatctaca atcaaagttg
361aagaatgcaa aatggctggc aaagatccca cacacggctg tgggaacttt gtccgtgtaa
421ttcagacttt caatcgcaca catttgtatg tctgtgggag tggcgctttc agtcctgtct
481gtacttactt gaacagaggg aggagatcag aggaccaagt tttcatgatt gactccaagt
541gtgaatctgg aaaaggacgc tgctctttca accccaacgt gaacacggtg tctgttatga
601tcaatgagga gcttttctct ggaatgtata tagatttcat ggggacagat gctgctattt
661ttcgaagttt aaccaagagg aatgcggtca gaactgatca acataattcc aaatggctaa
721gtgaacctat gtttgtagat gcacatgtca tcccagatgg tactgatcca aatgatgcta
781aggtgtactt cttcttcaaa gaaaaactga ctgacaataa caggagcacg aaacagattc
841attccatgat tgctcgaata tgtcctaatg acactggtgg actgcgtagc cttgtcaaca
901agtggaccac tttcttaaag gcgaggctgg tgtgctcggt aacagatgaa gacggcccag
961aaacacactt tgatgaatta gaggatgtgt ttctgctgga aactgataac ccgaggacaa
1021cactagtgta tggcattttt acaacatcaa gctcagtttt caaaggatca gccgtgtgtg
1081tgtatcattt atctgatata cagactgtgt ttaatgggcc ttttgcccac aaagaagggc
1141ccaatcatca gctgatttcc tatcagggca gaattccata tcctcgccct ggaacttgtc
1201caggaggagc atttacaccc aatatgcgaa ccaccaagga gttcccagat gatgttgtca
1261cttttattcg gaaccatcct ctcatgtaca attccatcta cccaatccac aaaaggcctt
1321tgattgttcg tattggcact gactacaagt acacaaagat agctgtggat cgagtgaacg
1381ctgctgatgg gagataccat gtcctgtttc tcggaacaga tcggggtact gtgcaaaaag
1441tggttgttct tcctactaac aactctgtca gtggcgagct cattctggag gagctggaag
1501tctttaagaa tcatgctcct ataacaacaa tgaaaatttc atctaaaaag caacagttgt
1561atgtgagttc caatgaaggg gtttcccagg tatctctgca ccgctgccac atctatggta
1621cagcctgtgc tgactgctgc ctggcgcggg acccttattg cgcctgggat ggccattcct
1681gttccagatt ctacccaact gggaaacgga ggagccgaag acaagatgtg agacatggaa
1741acccactgac tcaatgcaga ggatttaatc taaaagcata cagaaatgca gctgaaattg
1801tgcagtatgg agtaaaaaat aacaccactt ttctggagtg tgcccccaag tctccgcagg
1861catctatcaa gtggctgtta cagaaagaca aagacaggag gaaagaggtt aagctgaatg
1921aacgaataat agccacttca cagggactcc tgatccgctc tgttcagggt tctgaccaag
1981gactttatca ctgcattgct acagaaaata gtttcaagca gaccatagcc aagatcaact
2041tcaaagtttt agattcagaa atggtggctg ttgtGACGGA CAAATGGTCC CCGTGGACCT
2101GGGCCAGCTC TGTGAGGGCT TTACCCTTCC ACCCGAAGGA CATCATGGGG GCATTCAGCC
2161ACTCAGAAAT GCAGATGATT AACCAATACT GCAAAGACAC TCGGCAGCAA CATCAGCAGG
2221GAGATGAATC ACAGAAAATG AGAGGGGACT ATGGCAAGTT AAAGGCCCTC ATCAATAGTC
2281GGAAAAGTAG AAACAGGAGG AATCAGTTGC CAGAGTCATA CCCAACTTTC TTGTACAAAG
2341TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2401GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2461ACGAAGACAT CTAGTTTTCC TGGCCAAGAC GCGTTAAGTC gacaatcaac ctctggatta
2521caaaatttgt gaaagatt

Download FASTA (ORF) (Full)