Construct: ORF TRCN0000473430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008960.1_s317c1
- Derived from:
- ccsbBroadEn_07625
- DNA Barcode:
- AGACATCTAGTTTTCCTGGCCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA3C (10512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473430
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10512 | SEMA3C | semaphorin 3C | NM_006379.5 | 99.8% | 100% | (many diffs) |
| 2 | human | 10512 | SEMA3C | semaphorin 3C | NM_001350120.2 | 97.4% | 97.6% | (many diffs) |
| 3 | human | 10512 | SEMA3C | semaphorin 3C | NM_001350121.1 | 92% | 92.2% | (many diffs) |
| 4 | mouse | 20348 | Sema3c | sema domain, immunoglobulin... | NM_013657.5 | 88.2% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2319
- ORF length:
- 2253
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc attccggaca atttgcgtgt tggttggagt atttatttgt tctatctgtg 121 tgaaaggatc ttcccagccc caagcaagag tttatttaac atttgatgaa cttcgagaaa 181 ccaagacctc tgaatacttc agcctttccc accatccttt agactacagg attttattaa 241 tggatgaaga tcaggaccgg atatatgtgg gaagcaaaga tcacattctt tccctgaata 301 ttaacaatat aagtcaagaa gctttgagtg ttttctggcc agcatctaca atcaaagttg 361 aagaatgcaa aatggctggc aaagatccca cacacggctg tgggaacttt gtccgtgtaa 421 ttcagacttt caatcgcaca catttgtatg tctgtgggag tggcgctttc agtcctgtct 481 gtacttactt gaacagaggg aggagatcag aggaccaagt tttcatgatt gactccaagt 541 gtgaatctgg aaaaggacgc tgctctttca accccaacgt gaacacggtg tctgttatga 601 tcaatgagga gcttttctct ggaatgtata tagatttcat ggggacagat gctgctattt 661 ttcgaagttt aaccaagagg aatgcggtca gaactgatca acataattcc aaatggctaa 721 gtgaacctat gtttgtagat gcacatgtca tcccagatgg tactgatcca aatgatgcta 781 aggtgtactt cttcttcaaa gaaaaactga ctgacaataa caggagcacg aaacagattc 841 attccatgat tgctcgaata tgtcctaatg acactggtgg actgcgtagc cttgtcaaca 901 agtggaccac tttcttaaag gcgaggctgg tgtgctcggt aacagatgaa gacggcccag 961 aaacacactt tgatgaatta gaggatgtgt ttctgctgga aactgataac ccgaggacaa 1021 cactagtgta tggcattttt acaacatcaa gctcagtttt caaaggatca gccgtgtgtg 1081 tgtatcattt atctgatata cagactgtgt ttaatgggcc ttttgcccac aaagaagggc 1141 ccaatcatca gctgatttcc tatcagggca gaattccata tcctcgccct ggaacttgtc 1201 caggaggagc atttacaccc aatatgcgaa ccaccaagga gttcccagat gatgttgtca 1261 cttttattcg gaaccatcct ctcatgtaca attccatcta cccaatccac aaaaggcctt 1321 tgattgttcg tattggcact gactacaagt acacaaagat agctgtggat cgagtgaacg 1381 ctgctgatgg gagataccat gtcctgtttc tcggaacaga tcggggtact gtgcaaaaag 1441 tggttgttct tcctactaac aactctgtca gtggcgagct cattctggag gagctggaag 1501 tctttaagaa tcatgctcct ataacaacaa tgaaaatttc atctaaaaag caacagttgt 1561 atgtgagttc caatgaaggg gtttcccagg tatctctgca ccgctgccac atctatggta 1621 cagcctgtgc tgactgctgc ctggcgcggg acccttattg cgcctgggat ggccattcct 1681 gttccagatt ctacccaact gggaaacgga ggagccgaag acaagatgtg agacatggaa 1741 acccactgac tcaatgcaga ggatttaatc taaaagcata cagaaatgca gctgaaattg 1801 tgcagtatgg agtaaaaaat aacaccactt ttctggagtg tgcccccaag tctccgcagg 1861 catctatcaa gtggctgtta cagaaagaca aagacaggag gaaagaggtt aagctgaatg 1921 aacgaataat agccacttca cagggactcc tgatccgctc tgttcagggt tctgaccaag 1981 gactttatca ctgcattgct acagaaaata gtttcaagca gaccatagcc aagatcaact 2041 tcaaagtttt agattcagaa atggtggctg ttgtGACGGA CAAATGGTCC CCGTGGACCT 2101 GGGCCAGCTC TGTGAGGGCT TTACCCTTCC ACCCGAAGGA CATCATGGGG GCATTCAGCC 2161 ACTCAGAAAT GCAGATGATT AACCAATACT GCAAAGACAC TCGGCAGCAA CATCAGCAGG 2221 GAGATGAATC ACAGAAAATG AGAGGGGACT ATGGCAAGTT AAAGGCCCTC ATCAATAGTC 2281 GGAAAAGTAG AAACAGGAGG AATCAGTTGC CAGAGTCATA CCCAACTTTC TTGTACAAAG 2341 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2401 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2461 ACGAAGACAT CTAGTTTTCC TGGCCAAGAC GCGTTAAGTC gacaatcaac ctctggatta 2521 caaaatttgt gaaagatt