Construct: ORF TRCN0000473431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000612.1_s317c1
- Derived from:
- ccsbBroadEn_06557
- DNA Barcode:
- GGTCCTACTTCGACATAAGTCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAOA (4128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4128 | MAOA | monoamine oxidase A | NM_000240.3 | 99.9% | 100% | 1576C>A |
| 2 | human | 4128 | MAOA | monoamine oxidase A | NM_001270458.1 | 74.6% | 74.7% | 0_1ins399;1177C>A |
| 3 | mouse | 17161 | Maoa | monoamine oxidase A | NM_173740.3 | 84.6% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1647
- ORF length:
- 1581
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaatcaagag aaggcgagta tcgcgggcca catgttcgac gtagtcgtga 121 tcggaggtgg catttcagga ctatctgctg ccaaactctt gactgaatat ggcgttagtg 181 ttttggtttt agaagctcgg gacagggttg gaggaagaac atatactata aggaatgagc 241 atgttgatta cgtagatgtt ggtggagctt atgtgggacc aacccaaaac agaatcttac 301 gcttgtctaa ggagctgggc atagagactt acaaagtgaa tgtcagtgag cgtctcgttc 361 aatatgtcaa ggggaaaaca tatccatttc ggggcgcctt tccaccagta tggaatccca 421 ttgcatattt ggattacaat aatctgtgga ggacaataga taacatgggg aaggagattc 481 caactgatgc accctgggag gctcaacatg ctgacaaatg ggacaaaatg accatgaaag 541 agctcattga caaaatctgc tggacaaaga ctgctaggcg gtttgcttat ctttttgtga 601 atatcaatgt gacctctgag cctcacgaag tgtctgccct gtggttcttg tggtatgtga 661 agcagtgcgg gggcaccact cggatattct ctgtcaccaa tggtggccag gaacggaagt 721 ttgtaggtgg atctggtcaa gtgagcgaac ggataatgga cctcctcgga gaccaagtga 781 agctgaacca tcctgtcact cacgttgacc agtcaagtga caacatcatc atagagacgc 841 tgaaccatga acattatgag tgcaaatacg taattaatgc gatccctccg accttgactg 901 ccaagattca cttcagacca gagcttccag cagagagaaa ccagttaatt cagcggcttc 961 caatgggagc tgtcattaag tgcatgatgt attacaagga ggccttctgg aagaagaagg 1021 attactgtgg ctgcatgatc attgaagatg aagatgctcc aatttcaata accttggatg 1081 acaccaagcc agatgggtca ctgcctgcca tcatgggctt cattcttgcc cggaaagctg 1141 atcgacttgc taagctacat aaggaaataa ggaagaagaa aatctgtgag ctctatgcca 1201 aagtgctggg atcccaagaa gctttacaTC CAGTGCATTA TGAAGAGAAG AACTGGTGTG 1261 AGGAGCAGTA CTCTGGGGGC TGCTACACGG CCTACTTCCC TCCTGGGATC ATGACTCAAT 1321 ATGGAAGGGT GATTCGTCAA CCCGTGGGCA GGATTTTCTT TGCGGGCACA GAGACTGCCA 1381 CAAAGTGGAG CGGCTACATG GAAGGGGCAG TTGAGGCTGG AGAACGAGCA GCTAGGGAGG 1441 TCTTAAATGG TCTCGGGAAG GTGACCGAGA AAGATATCTG GGTACAAGAA CCTGAATCAA 1501 AGGACGTTCC AGCGGTAGAA ATCACCCACA CCTTCTGGGA AAGGAACCTG CCCTCTGTTT 1561 CTGGCCTGCT GAAGATCATT GGATTTTCCA CATCAGTAAC TGCCCTGGGG TTTGTGCTGT 1621 ACAAATACAA GCTCCTGCCA AGGTCTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1681 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1741 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGTCCTAC 1801 TTCGACATAA GTCGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1861 aagatt