Construct: ORF TRCN0000473436
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003022.1_s317c1
- Derived from:
- ccsbBroadEn_05937
- DNA Barcode:
- TATCCTTAAGGATGAAGAATGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASP7 (840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473436
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 840 | CASP7 | caspase 7 | NM_001227.5 | 99.8% | 99.6% | 12T>G |
| 2 | human | 840 | CASP7 | caspase 7 | NM_001267056.1 | 99.8% | 99.6% | 12T>G |
| 3 | human | 840 | CASP7 | caspase 7 | NM_033339.5 | 99.8% | 99.6% | 12T>G |
| 4 | human | 840 | CASP7 | caspase 7 | NM_001320911.2 | 95.8% | 86.5% | 0_1insATGGCAG;5T>G;101_131del |
| 5 | human | 840 | CASP7 | caspase 7 | XM_017016764.1 | 95.8% | 86.5% | 0_1insATGGCAG;5T>G;101_131del |
| 6 | human | 840 | CASP7 | caspase 7 | XM_006718017.3 | 95.4% | 84.9% | 1_11del;23T>G;119_149del |
| 7 | human | 840 | CASP7 | caspase 7 | XM_017016763.1 | 93.9% | 93.7% | 1_57del;69T>G |
| 8 | human | 840 | CASP7 | caspase 7 | NM_001267058.1 | 90.8% | 88.7% | (many diffs) |
| 9 | human | 840 | CASP7 | caspase 7 | NM_033338.6 | 90% | 89.8% | 1_99del;111T>G |
| 10 | human | 840 | CASP7 | caspase 7 | XM_011540260.1 | 88.8% | 88.1% | 1_2delATins89;7_8insTACCGTCCCTCT |
| 11 | human | 840 | CASP7 | caspase 7 | NM_033340.4 | 79.6% | 49.1% | (many diffs) |
| 12 | human | 840 | CASP7 | caspase 7 | NM_001267057.1 | 78% | 69.4% | 1_224del;236T>G;332_362del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agatgagcag ggctgtattg aagagcaggg ggttgaggat tcagcaaatg 121 aagattcagt ggatgctaag ccagaccggt cctcgtttgt accgtccctc ttcagtaaga 181 agaagaaaaa tgtcaccatg cgatccatca agaccacccg ggaccgagtg cctacatatc 241 agtacaacat gaattttgaa aagctgggca aatgcatcat aataaacaac aagaactttg 301 ataaagtgac aggtatgggc gttcgaaacg gaacagacaa agatgccgag gcgctcttca 361 agtgcttccg aagcctgggt tttgacgtga ttgtctataa tgactgctct tgtgccaaga 421 tgcaagatct gcttaaaaaa gcttctgaag aggaccatac aaatgccgcc tgcttcgcct 481 gcatccTCTT AAGCCATGGA GAAGAAAATG TAATTTATGG GAAAGATGGT GTCACACCAA 541 TAAAGGATTT GACAGCCCAC TTTAGGGGGG ATAGATGCAA AACCCTTTTA GAGAAACCCA 601 AACTCTTCTT CATTCAGGCT TGCCGAGGGA CCGAGCTTGA TGATGGCATC CAGGCCGACT 661 CGGGGCCCAT CAATGACACA GATGCTAATC CTCGATACAA GATCCCAGTG GAAGCTGACT 721 TCCTCTTCGC CTATTCCACG GTTCCAGGCT ATTACTCGTG GAGGAGCCCA GGAAGAGGCT 781 CCTGGTTTGT GCAAGCCCTC TGCTCCATCC TGGAGGAGCA CGGAAAAGAC CTGGAAATCA 841 TGCAGATCCT CACCAGGGTG AATGACAGAG TTGCCAGGCA CTTTGAGTCT CAGTCTGATG 901 ACCCACACTT CCATGAGAAG AAGCAGATCC CCTGTGTGGT CTCCATGCTC ACCAAGGAAC 961 TCTACTTCAG TCAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATCCTTAAG GATGAAGAAT 1141 GATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt