Construct: ORF TRCN0000473449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005539.1_s317c1
- Derived from:
- ccsbBroadEn_01691
- DNA Barcode:
- ACCGGCGGCTGCCCGCTGTTTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNNI3 (7137)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473449
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7137 | TNNI3 | troponin I3, cardiac type | NM_000363.5 | 100% | 100% | |
| 2 | mouse | 21954 | Tnni3 | troponin I, cardiac 3 | NM_009406.4 | 88.6% | 92.4% | (many diffs) |
| 3 | mouse | 21954 | Tnni3 | troponin I, cardiac 3 | XM_017322110.1 | 85.3% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 699
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggatggg agcagcgatg cggctaggga acctcgccct gcaccagccc 121 caatcagacg ccgctcctcc aactaccgcg cttatgccac GGAGCCGCAC GCCAAGAAAA 181 AATCTAAGAT CTCCGCCTCG AGAAAATTGC AGCTGAAGAC TCTGCTGCTG CAGATTGCAA 241 AGCAAGAGCT GGAGCGAGAG GCGGAGGAGC GGCGCGGAGA GAAGGGGCGC GCTCTGAGCA 301 CCCGCTGCCA GCCGCTGGAG TTGGCCGGGC TGGGCTTCGC GGAGCTGCAG GACTTGTGCC 361 GACAGCTCCA CGCCCGTGTG GACAAGGTGG ATGAAGAGAG ATACGACATA GAGGCAAAAG 421 TCACCAAGAA CATCACGGAG ATTGCAGATC TGACTCAGAA GATCTTTGAC CTTCGAGGCA 481 AGTTTAAGCG GCCCACCCTG CGGAGAGTGA GGATCTCTGC AGATGCCATG ATGCAGGCGC 541 TGCTGGGGGC CCGGGCTAAG GAGTCCCTGG ACCTGCGGGC CCACCTCAAG CAGGTGAAGA 601 AGGAGGACAC CGAGAAGGAA AACCGGGAGG TGGGAGACTG GCGCAAGAAC ATCGATGCAC 661 TGAGTGGAAT GGAGGGCCGC AAGAAAAAGT TTGAGAGCTT GCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGAACCGGC GGCTGCCCGC TGTTTTGCAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt