Construct: ORF TRCN0000473454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017599.1_s317c1
- Derived from:
- ccsbBroadEn_06915
- DNA Barcode:
- GAACTTTGTCAAGCAAGGTATCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCNN1B (6338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473454
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6338 | SCNN1B | sodium channel epithelial 1... | NM_000336.3 | 99.9% | 99.8% | 122T>C |
| 2 | human | 6338 | SCNN1B | sodium channel epithelial 1... | XM_011545914.1 | 99% | 98.9% | 1_18del;140T>C |
| 3 | human | 6338 | SCNN1B | sodium channel epithelial 1... | XM_011545913.2 | 98.2% | 98.1% | 1_33del;155T>C |
| 4 | human | 6338 | SCNN1B | sodium channel epithelial 1... | XM_017023525.1 | 97% | 96.9% | 1_57del;179T>C |
| 5 | human | 6338 | SCNN1B | sodium channel epithelial 1... | XM_017023526.1 | 91.6% | 91.5% | 1_57del;179T>C;1100_1101ins108 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1986
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cgtgaagaag tacctgctga agggcctgca tcggctgcag aagggccccg 121 gctacacgta caaggagctg ctggtgtggt actgcgacaa caccaacacc cacggcccca 181 agcgcaccat ctgtgagggg cccaagaaga aagccatgtg gttcctgctc accctgctct 241 tcgccgccct cgtctgctgg cagtggggca tcttcatcag gacctacttg agctgggagg 301 tcagcgtctc cctctccgta ggcttcaaga ccatggactt ccctgccgtc accatctgca 361 atgctagccc cttcaagtat tccaaaatca agcatttgct gaaggacctg gatgagctga 421 tggaagctgt cctggagaga atcctggctc ctgagctaag ccatgccaat gccaccagga 481 acctgaactt ctccatctgg aaccacacac ccctggtcct tattgatgaa cggaaccccc 541 accaccccat ggtccttgat ctctttggag acaaccacaa tggcttaaca agcagctcag 601 catcagaaaa gatctgtaat gcccacgggt gcaaaatggc catgagacta tgtagcctca 661 acaggaccca gtgtaccttc cggaacttca ccagtgctac ccaggcattg acagagtggt 721 acatcctgca ggccaccaac atctttgcac aggtgccaca gcaggagcta gtagagatga 781 gctaccccgg cgagcagatg atcctggcct gcctattcgg agctgagccc tgcaactacc 841 ggaacttcac gtccatcttc taccctcact atggcaactg ttacatcttc aactggggca 901 tgacagagaa ggcacttcct tcggccaacc ctggaactga attcggcctg aagttgatcc 961 tggacatagg ccaggaagac tacgtcccct tccttgcgtc cacggccggg gtcaggctga 1021 tgcttcacga gcagaggtca taccccttca tcagagatga gggcatctac gccatgtcgg 1081 ggacagagac gtccatcggg gtactcgtgg acaagcttca gcgcatgggg gagccctaca 1141 gcccgtgcac cgtgaatggt tctgaggtcc ccgtccaaaa cttctacagt gactacaaca 1201 cgacctactc catccaggcc tgtcttcgct cctgcttcca agaccacatg atccgtaact 1261 gcaactgtgg ccactacctg tacccactgc cccgtgggga gaaatactgc aacaaccggg 1321 acttcccaga ctgggcccat tgctactcag atctacagat gagcgtggcg cagagagaga 1381 cctgcattgg catgtgcaag gagtcctgca atgacaccca gtacaagatg accatctcca 1441 tggctgactg gccttctgag gcctccgagg actggatttt ccacgtcttg tctcaggagc 1501 gggaccaaag caccaatatc acccTGAGCA GGAAGGGAAT TGTCAAGCTC AACATCTACT 1561 TCCAAGAATT TAACTATCGC ACCATTGAAG AATCAGCAGC CAATAACATC GTCTGGCTGC 1621 TCTCGAATCT GGGTGGCCAG TTTGGCTTCT GGATGGGGGG CTCTGTGCTG TGCCTCATCG 1681 AGTTTGGGGA GATCATCATC GACTTTGTGT GGATCACCAT CATCAAGCTG GTGGCCTTGG 1741 CCAAGAGCCT ACGGCAGCGG CGAGCCCAAG CCAGCTACGC TGGCCCACCG CCCACCGTGG 1801 CCGAGCTGGT GGAGGCCCAC ACCAACTTTG GCTTCCAGCC TGACACGGCC CCCCGCAGCC 1861 CCAACACTGG GCCCTACCCC AGTGAGCAGG CCCTGCCCAT CCCAGGCACC CCGCCCCCCA 1921 ACTATGACTC CCTGCGTCTG CAGCCGCTGG ACGTCATCGA GTCTGACAGT GAGGGTGATG 1981 CCATCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2041 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2101 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGAACTTTGT CAAGCAAGGT ATCGCACGCG 2161 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt