Construct: ORF TRCN0000473457
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014081.1_s317c1
- Derived from:
- ccsbBroadEn_00913
- DNA Barcode:
- CCGAAACTCTGCGAGGCCGGTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF22 (3835)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473457
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3835 | KIF22 | kinesin family member 22 | NM_007317.3 | 99.8% | 100% | 1230C>T;1425G>A |
| 2 | human | 3835 | KIF22 | kinesin family member 22 | XR_243280.4 | 92.7% | (many diffs) | |
| 3 | human | 3835 | KIF22 | kinesin family member 22 | XM_024450270.1 | 91.8% | 86.6% | (many diffs) |
| 4 | human | 3835 | KIF22 | kinesin family member 22 | NM_001256269.2 | 89.6% | 89.7% | 0_1ins204;1026C>T;1221G>A |
| 5 | human | 3835 | KIF22 | kinesin family member 22 | NM_001256270.1 | 89.6% | 89.7% | 0_1ins204;1026C>T;1221G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2061
- ORF length:
- 1995
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcgggcggc tcgacgcagc agaggcgacg cgagatggcg gcagcttcag 121 cggcggcgat ctcaggagct ggtcgctgtc ggctaagcaa gattggagct actcgtcgtc 181 cacctccagc tcgcgtaagg gtggctgtgc gactgcggcc atttgtggat ggaacagcgg 241 gagcaagtga tcccccctgt gtgcggggca tggacagctg ctctctagag attgctaact 301 ggaggaacca ccaggagact ctcaaatacc agtttgatgc cttctatggg gagaggagta 361 ctcagcagga catctatgca ggttcagtgc agcccatcct aaggcacttg ctggaagggc 421 agaatgccag tgtgcttgcc tatggaccca caggagctgg gaagacgcac acaatgctgg 481 gcagcccaga gcaacctggg gtgatcccgc gggctctcat ggacctcctg cagctcacaa 541 gggaggaggg tgccgagggc cggccatggg ccctttctgt caccatgtct tacctagaga 601 tctaccagga gaaggtatta gacctcctgg accctgcttc gggagacctg gtaatccgag 661 aagactgccg ggggaatatc ctgattccgg gtctctccca gaagcccatc agtagctttg 721 ctgattttga gcggcacttc ctgccagcca gtcgaaatcg gactgtagga gccacccggc 781 tcaaccagcg ctcctcccgc agtcatgctg tgctcctggt caaggtggac cagcgggaac 841 gtttggcccc atttcgccag cgagagggaa aactctacct gattgacttg gctgggtcag 901 aggacaaccg gcgcacaggc aacaagggcc ttcggctaaa agagagtgga gccatcaaca 961 cctccctgtt tgtcctgggc aaagtggtag atgcgctgaa tcagggcctc cctcgtgtac 1021 cttatcggga cagcaagctc actcgcctat tgcaggactc tctgggtggc tcagcccaca 1081 gtatccttat tgccaacatt gcccctgaga gacgcttcta cctagacaca gtctccgcac 1141 tcaactttgc tgccaggtcc aaggaggtga tcaatcggcc ttttaccaat gagagcctgc 1201 agcctcatgc cttgggacct gttaagctgt ctcagaaaga attgcttggt ccaccagagg 1261 caaagagagc ccgaggccct gaggaagagg agattgggag ccctgagccc atggcagctc 1321 cagcctctgc ctcccagaaa ctcagccccc tacagaagct aagcagcatg gacccggcca 1381 tgctggagcg cctcctcagc ttggaccgtc tgcttgcctc ccaggggagc cagggggccc 1441 ctctgttgag taccccaaag cgagagcgga tggtgctaat gaagacagta gaagagaagg 1501 acctagagat tgagaggctt aagacgaagc aaaaagaact ggaggccaag atgttggccc 1561 agaaggctga ggaaaaggag aaccattgtc ccacaatgct ccggcccctt tcacatcgca 1621 cagtcacagg gGCAAAGCCC CTGAAAAAGG CTGTGGTGAT GCCCCTACAG CTAATTCAGG 1681 AGCAGGCAGC ATCCCCAAAT GCCGAGATCC ACATCCTGAA GAATAAAGGC CGGAAGAGAA 1741 AGCTGGAGTC CCTGGATGCC CTAGAGCCTG AGGAGAAGGC TGAGGACTGC TGGGAGCTAC 1801 AGATCAGCCC GGAGCTACTG GCTCATGGGC GCCAAAAAAT ACTGGATCTG CTGAACGAAG 1861 GCTCAGCCCG AGATCTCCGC AGTCTTCAGC GCATTGGCCC GAAGAAGGCC CAGCTAATCG 1921 TGGGCTGGCG GGAGCTCCAC GGCCCCTTCA GCCAGGTGGA GGACCTGGAA CGCGTGGAGG 1981 GCATAACGGG GAAACAGATG GAGTCCTTCC TGAAGGCAAA CATCCTGGGT CTCGCCGCCG 2041 GCCAGCGCTG TGGCGCCTCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2101 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2161 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGA AACTCTGCGA 2221 GGCCGGTAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt