Construct: ORF TRCN0000473470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007110.1_s317c1
- Derived from:
- ccsbBroadEn_06554
- DNA Barcode:
- ACACGAGCCGGCTATAACCGGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEB2 (4113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473470
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4113 | MAGEB2 | MAGE family member B2 | NM_002364.5 | 99.6% | 99.6% | 181G>A;216G>A;306C>T |
| 2 | human | 4113 | MAGEB2 | MAGE family member B2 | XM_011545512.1 | 99.6% | 99.6% | 181G>A;216G>A;306C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1023
- ORF length:
- 957
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcgtggtcag aagagtaagc tccgtgcccg tgagaaacgc cgcaaggccc 121 gagatgagac ccggggtctc aatgttcctc aggtcactga agcagaggaa gaagaggccc 181 cctgctgttc ctcttctgtt tctgggggtg ctgcttcaag ctctcctgct gctggcattc 241 cccagaagcc tcagagagcc ccaaccactg ccgctgctgc agctgcgggt gtttcatcca 301 caaaatctaa aaaaggtgcc aagagccacc aaggtgagaa aaatgcaagt tcctcccagg 361 cctcaacatc tactaagagc ccaagcgaag atcctctaac caggaagtca gggtcgttgg 421 tgcagttcct gttgtacaag tataaaataa aaaagtccgt tacaaaggga gaaatgctga 481 aaattgttgg caaaaggttc agggagcact tccctgagat cctcaagaaa gcctctgagg 541 gcctcagtgt tgtctttggc cttgagctga ataaagtcaa ccccaacggc cacacttaca 601 ccttcatcga caaggtagac ctcactgatg aggaatcccT GCTCAGTTCC TGGGACTTTC 661 CCAGGAGAAA GCTTCTGATG CCTCTCCTGG GTGTGATCTT CTTAAATGGC AACTCAGCTA 721 CTGAGGAAGA GATCTGGGAA TTCCTGAATA TGTTGGGAGT CTATGATGGA GAGGAGCACT 781 CAGTCTTTGG GGAACCCTGG AAGCTCATCA CCAAAGATCT GGTGCAGGAA AAATATCTGG 841 AGTACAAGCA GGTGCCCAGC AGTGATCCCC CACGCTTTCA ATTCCTGTGG GGTCCGAGAG 901 CCTATGCTGA AACCAGCAAG ATGAAAGTCC TGGAGTTTTT GGCCAAGGTA AATGGTACCA 961 CCCCCTGTGC CTTCCCAACC CATTACGAAG AAGCTTTGAA AGATGAAGAG AAAGCCGGAG 1021 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGAAC ACGAGCCGGC TATAACCGGG AAACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt