Construct: ORF TRCN0000473482
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003487.1_s317c1
- Derived from:
- ccsbBroadEn_02382
- DNA Barcode:
- CGAGACTATTGACCTATTAATTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DSCR4 (10281)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473482
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10281 | DSCR4 | Down syndrome critical regi... | NR_147130.1 | 31.7% | 1_105del;460_1114del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 444
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCGTTAAT CATCTTGACG AGAGATGATG 121 AACCCCGGAT ATTTACCCCA GACAGTGATG CCGCTTCACC AGCATTGCAC TCTACTTCCC 181 CGCTTCCTGA TCCTGCCTCA GCTTCTCCTC TCCACAGAGA AGAAAAAATT CTGCCTAAAG 241 TCTGCAACAT CGTTTCCTGC CTGAGTTTCA GCCTGCCAGC TTCTCCTACG GATTCTGGAC 301 TTGCCAGCCC CACAATCATA ACCAGAGAGG GGCAGCAATT TTGGGCAAAA TGTCTGATTT 361 GGAAATACCA ACTTTACCTC CATGGGCTCC ACAAGAAATC AGATGGGAGA AGGGACAAGC 421 AGATAAGCGC AAGCCCATCA ACCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GAGACTATTG 601 ACCTATTAAT TACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att