Construct: ORF TRCN0000473517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007084.1_s317c1
- Derived from:
- ccsbBroadEn_03368
- DNA Barcode:
- ACTTGCAAGCTACGAAACAAAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPRC5B (51704)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473517
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51704 | GPRC5B | G protein-coupled receptor ... | NM_016235.3 | 100% | 100% | |
| 2 | human | 51704 | GPRC5B | G protein-coupled receptor ... | XM_006721051.3 | 100% | 100% | |
| 3 | human | 51704 | GPRC5B | G protein-coupled receptor ... | XM_006721052.2 | 100% | 100% | |
| 4 | human | 51704 | GPRC5B | G protein-coupled receptor ... | NM_001304771.1 | 75.4% | 75.4% | 1_393del |
| 5 | mouse | 64297 | Gprc5b | G protein-coupled receptor,... | NM_022420.2 | 84.8% | 85.6% | (many diffs) |
| 6 | mouse | 64297 | Gprc5b | G protein-coupled receptor,... | NM_001195774.1 | 82.1% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgtggcatca gagagaaaga tgagagctca ccaggtgctc accttcctcc 121 tgctcttcgt gatcacctcg gtggcctctg aaaacgccag cacatcccga ggctgtgggc 181 tggacctcct ccctcagtac gtgtccctgt gcgacctgga cgccatctgg ggcattgtgg 241 tggaggcggt ggccggggcg ggcgccctga tcacactgct cctgatgctc atcctcctgg 301 tgcggctgcc cttcatcaag gagaaggaga agaagagccc tgtgggcctc cactttctgt 361 tcctcctggg gaccctgggc ctctttgggc tgacgtttgc cttcatcatc caggaggacg 421 agaccatctg ctctgtccgc cgcttcctct ggggcgtcct ctttgcgctc tgcttctcct 481 gcctgctgag ccaggcatgg cgcgtgcgga ggctggtgcg gcatggcacg ggccccgcgg 541 gctggcagct ggtgggcctg gcgctgtgcc tgatgctggt gcaagtcatc atcgctgtgg 601 agtggctggt gctcaccgtg ctgcgtgaca caaggccagc ctgcgcctac gagcccatgg 661 actttgtgat ggccctcatc tacgacatgg tactgcttgt ggtcaccctg gggctggccc 721 tcttcactct gtgcggcaag ttcaagaggt ggaagctgaa cggggccttc ctcctcatca 781 cagccttcct ctctgtgctc atctgggtgg cctGGATGAC CATGTACCTC TTCGGCAATG 841 TCAAGCTGCA GCAGGGGGAT GCCTGGAACG ACCCCACCTT GGCCATCACG CTGGCGGCCA 901 GCGGCTGGGT CTTCGTCATC TTCCACGCCA TCCCTGAGAT CCACTGCACC CTTCTGCCAG 961 CCCTGCAGGA GAACACGCCC AACTACTTCG ACACGTCGCA GCCCAGGATG CGGGAGACGG 1021 CCTTCGAGGA GGACGTGCAG CTGCCGCGGG CCTATATGGA GAACAAGGCC TTCTCCATGG 1081 ATGAACACAA TGCAGCTCTC CGAACAGCAG GATTTCCCAA CGGCAGCTTG GGAAAAAGAC 1141 CCAGTGGCAG CTTGGGGAAA AGACCCAGCG CTCCGTTTAG AAGCAACGTG TATCAGCCAA 1201 CTGAGATGGC CGTCGTGCTC AACGGTGGGA CCATCCCAAC TGCTCCGCCA AGTCACACAG 1261 GAAGACACCT TTGGTGCTCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACTTGCAAGC TACGAAACAA 1441 AGACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt