Construct: ORF TRCN0000473521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000266.1_s317c1
- Derived from:
- ccsbBroadEn_14056
- DNA Barcode:
- GATCGCTATGAACCGCGTCGTGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CYSLTR1 (10800)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282186.1 | 99.6% | 66.2% | (many diffs) |
| 2 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282187.2 | 99.6% | 66.2% | (many diffs) |
| 3 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282188.2 | 99.6% | 66.2% | (many diffs) |
| 4 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_006639.4 | 99.6% | 66.2% | (many diffs) |
| 5 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_001281862.1 | 87.1% | 57.3% | (many diffs) |
| 6 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_001281859.1 | 83.9% | 55.2% | (many diffs) |
| 7 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_021476.5 | 83.9% | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgaaactgga aatctgacag tatcttctgc cacatgccat gacactattg 121 atgacttccg caatcaagtg tattccacct tgtactctat gatctctgtt gtaggcttct 181 ttggcaatgg ctttgtgctc tatgtcctca taaaaaccta tcacaagaag tcagccttcc 241 aagtatacat gattaattta gcagtagcag atctactttg tgtgtgcaca ctgcctctcc 301 gtgtggtcta ttatgttcac aaaggcattt ggctctttgg tgacttcttg tgccgcctca 361 gcacctatgc tttgtatgtc aacctctatt gtagcatctt ctttatgaca gccatgagct 421 ttttccggtg cattgcaatt gtttttccag tccagaacat taatttggtt acacagaaaa 481 aagccaggtt tgtgtgtgta ggtatttgga tttttgtgat tttgaccagt tctccatttc 541 taatggccaa accacaaaaa gatgagaaaa ataataccaa gtgctttgag cccccacaag 601 acaatcaaac taaaaatcat gttttggtct tgcattatgt gtcattgttt gttggcttta 661 tcatcccttt tgttattata attgtctgtt acacaatgat cattttgacc ttactaaaaa 721 aatcaatgan aaaaaaatct gtcaagtcat aaaaaggcta taggaatgat catggtcgtg 781 accgctgcct ttttagtcag tttcatgcca tatcatattc aacgtaccat tcaccTTCAT 841 TTTTTACACA ATGAAACTAA ACCCTGTGAT TCTGTCCTTA CAATGCAGAA GTCCGTGGTC 901 ATAACCTTGT CTCTGGCTGC ATCCAATTGT TGCTTTGACC CTCTCCTATA TTTCTTTTCT 961 GGGGGTAACT TTAGGAAAAG GCTGTCTACA TTTAGAAAGC ATTCTTTGTC CAGCGTGACT 1021 TATGTACCCA GAAAGAAGGC CTCTTTGCCA GAAAAAGGAG AAGAAATATG TAAAGTATAC 1081 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1141 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1201 TATATATCTT GTGGAAAGGA CGAGATCGCT ATGAACCGCG TCGTGTCACG CGTTAAGTCg 1261 acaatcaacc tctggattac aaaatttgtg aaagatt