Construct: ORF TRCN0000473576
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003391.1_s317c1
- Derived from:
- ccsbBroadEn_14712
- DNA Barcode:
- GCGACGAGCCTGGGTGGATCACAA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFIA (4774)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473576
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4774 | NFIA | nuclear factor I A | NM_005595.5 | 68% | 67.8% | (many diffs) |
| 2 | human | 4774 | NFIA | nuclear factor I A | NM_001134673.4 | 66.5% | 66.4% | (many diffs) |
| 3 | human | 4774 | NFIA | nuclear factor I A | XM_017001362.2 | 66.2% | 66% | (many diffs) |
| 4 | human | 4774 | NFIA | nuclear factor I A | XM_011541515.3 | 65.6% | 65.5% | (many diffs) |
| 5 | human | 4774 | NFIA | nuclear factor I A | NM_001145511.2 | 64.8% | 64.6% | (many diffs) |
| 6 | human | 4774 | NFIA | nuclear factor I A | XM_011541512.3 | 64.2% | 64.1% | (many diffs) |
| 7 | human | 4774 | NFIA | nuclear factor I A | XM_011541514.3 | 62.6% | 62.4% | (many diffs) |
| 8 | human | 4774 | NFIA | nuclear factor I A | NM_001145512.2 | 61.1% | 61% | (many diffs) |
| 9 | human | 4774 | NFIA | nuclear factor I A | XM_017001363.1 | 15.6% | 15.5% | (many diffs) |
| 10 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502845.2 | 68.6% | 70.5% | (many diffs) |
| 11 | mouse | 18027 | Nfia | nuclear factor I/A | NM_001122953.1 | 67% | 68.8% | (many diffs) |
| 12 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502843.2 | 63.8% | 67.2% | (many diffs) |
| 13 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502844.3 | 63.2% | 64.2% | (many diffs) |
| 14 | mouse | 18027 | Nfia | nuclear factor I/A | NM_010905.3 | 62.4% | 65.8% | (many diffs) |
| 15 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502842.3 | 60.7% | 64% | (many diffs) |
| 16 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502841.3 | 60.4% | 62.9% | (many diffs) |
| 17 | mouse | 18027 | Nfia | nuclear factor I/A | NM_001122952.1 | 59.2% | 61.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ttctccgctc tgtctcaccc aggatgaatt tcatcctttc atcgaagcac 121 ttctgcccca cgtccgagcc tttgcctaca catggttcaa cctgcaggcc cgaaaacgaa 181 aatacttcaa aaaacatgaa aagcgtatgt caaaagaaga agagagagcc gtgaaggatg 241 aattgctaag tgaaaaacca gaggtcaagc agaagtgggc atctcgactt ctggcaaagt 301 tgcggaaaga tatccgaccc gaatatcgag aggattttgt tcttacagtt acagggaaaa 361 aacctccatg ttgtgttctt tccaacccag accagaaagg caagatgcga agaattgact 421 gcctccgcca ggcagataaa gtctggaggt tggaccttgt tatggtgatt ttgtttaaag 481 gtattccgct ggaaagtact gatggcgagc gccttgtaaa gtccccacaa tgctcTAATC 541 CAGGGCTCTG TGTCCAACCC CATCACATAG GGGTTTCTGT TAAGGAACTC GATTTATATT 601 TGGCATACTT TGTGCATGCA GCAGATTCAA GTCAATCTGA AAGTCCCAGC CAGCCAAGTG 661 ACGCTGACAT TAAGGACCAG CCAGAAAATG GACATTTGGG CTTCCAGGAC AGTTTTGTCA 721 CATCAGGTGT TTTTAGTGTC ACTGAGCTAG TAAGAGTGTC ACAGACACCA ATAGCTGCAG 781 GAACTGGCCC AAATTTTTCT CTCTCAGATT TGGAAAGTTC TTCATACTAC AGCATGAGTC 841 CAGGAGCAAT GAGGAGGTCT TTACCCAGCA CATCCTCTAC GAGCTCCACA AAGCGCCTCA 901 AGTCTGTGGA GGATGAAATG GACAGTCCTG GTGAGGAGCC ATTTTATACA GGCCAAGGGC 961 GCTCCCCAGG AAGTGGCAGT CAGTCAAGTG GATGGCATGA AGTGGAGCCA GGAATGCCAT 1021 CTCCAACCAC ACTGAAGAAG TCGGAGAAGT CTGGTTTCAG CAGCCCCTCC CCTTCTCAAC 1081 TCTGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 1141 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 1201 TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCGACGAGCC TGGGTGGATC ACAAACGCGT 1261 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt