Construct: ORF TRCN0000473577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000054.1_s317c1
- Derived from:
- ccsbBroadEn_08655
- DNA Barcode:
- TTACCCTCATTTCCATAGGCGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPYSL5 (56896)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473577
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56896 | DPYSL5 | dihydropyrimidinase like 5 | NM_001253723.1 | 99.9% | 100% | 9C>T |
| 2 | human | 56896 | DPYSL5 | dihydropyrimidinase like 5 | NM_001253724.1 | 99.9% | 100% | 9C>T |
| 3 | human | 56896 | DPYSL5 | dihydropyrimidinase like 5 | NM_020134.4 | 99.9% | 100% | 9C>T |
| 4 | human | 56896 | DPYSL5 | dihydropyrimidinase like 5 | XM_024453007.1 | 99.9% | 100% | 9C>T |
| 5 | human | 56896 | DPYSL5 | dihydropyrimidinase like 5 | XR_001738852.2 | 30.5% | (many diffs) | |
| 6 | mouse | 65254 | Dpysl5 | dihydropyrimidinase-like 5 | NM_023047.2 | 90.3% | 97.8% | (many diffs) |
| 7 | mouse | 65254 | Dpysl5 | dihydropyrimidinase-like 5 | XM_006504045.1 | 90.3% | 97.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1758
- ORF length:
- 1692
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tgctaactca gccagcgtga ggatcctcat caagggaggc aaggtggtga 121 acgatgactg cacccacgag gctgacgtct acatcgagaa tggcatcatc cagcaggtgg 181 gccgcgagct catgatccct ggcggggcca aggtgattga tgccacagga aaactggtga 241 tccctggtgg catcgacacc agcacccact tccaccagac cttcatgaat gccacgtgcg 301 tggacgactt ctaccatggg accaaggcag cactcgtcgg aggcaccacc atgatcatcg 361 gccacgtcct gcccgacaag gagacctccc ttgtggacgc ttatgagaag tgccgaggtc 421 tggccgaccc caaggtctgc tgtgattacg ccctccacgt ggggatcacc tggtgggcac 481 ccaaggtgaa agcagaaatg gagacactgg tgagggagaa gggtgtcaac tcgttccaga 541 tgttcatgac ctacaaggac ctgtacatgc ttcgagacag tgagctgtac caagtgttgc 601 acgcttgcaa ggacattggg gcaatcgccc gcgtccatgc tgaaaatggg gagcttgtgg 661 ccgagggtgc taaggaggca ctggatttgg ggatcacagg cccagaagga atcgagatca 721 gccgtccaga ggagctggaa gctgaagcca ctcatcgtgt tatcaccatt gcaaacagga 781 ctcactgtcc aatctacctg gtcaacgtgt ccagtatctc ggctggtgac gttatcgcag 841 ctgctaagat gcaagggaag gttgtgctgg cggagaccac cactgcacat gccacgctga 901 caggcttaca ctactaccac caggactggt cccacgcggc tgcctatgtc acggtgcctc 961 ccctgagact ggacaccaac acctcaacct acctcatgag cctgctggcc aatgacactc 1021 tgaacatcgt ggcatcagat caccggcctt tcaccacaaa gcagaaagct atgggcaagg 1081 aagacttcac caagatccca catggagtga gtggcgtgca ggaccgcatg agcgtcatct 1141 gggagagagg agtggttgga ggaaagatgg atgagaaccg ttttgtggcc gttaccagtt 1201 ccaacgcagc taagcttctg aacctgtatc cccgcaaggg ccgcattatt cccggagccg 1261 atgctgatgt ggtggtgtgg gacccagaag ccacaaagaC CATCTCAGCC AGCACGCAGG 1321 TCCAGGGAGG AGACTTCAAC CTGTATGAGA ACATGCGCTG CCACGGCGTG CCACTGGTCA 1381 CCATCAGCCG GGGGCGCGTC GTGTATGAGA ACGGCGTCTT CATGTGCGCC GAGGGCACCG 1441 GCAAGTTCTG TCCCCTGAGG TCCTTCCCAG ACACTGTCTA CAAGAAGCTG GTCCAGAGAG 1501 AGAAGACTTT AAAGGTTAGA GGAGTGGACC GCACTCCCTA CCTGGGGGAT GTCGCTGTTG 1561 TCGTGCACCC TGGGAAAAAA GAGATGGGAA CCCCACTCGC AGACACTCCT ACCCGGCCCG 1621 TCACCCGGCA TGGGGGCATG AGGGACCTTC ACGAATCCAG CTTCAGCCTC TCTGGCTCTC 1681 AGATCGATGA CCATGTTCCA AAGCGAGCTT CAGCTCGGAT CCTCGCTCCT CCCGGAGGCA 1741 GGTCGAGTGG CATTTGGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1801 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1861 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTACCCT CATTTCCATA 1921 GGCGGGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt