Construct: ORF TRCN0000473589
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009294.1_s317c1
- Derived from:
- ccsbBroadEn_11004
- DNA Barcode:
- TTACAAAGCAGGGAAGAGGAGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NT5E (4907)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473589
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4907 | NT5E | 5'-nucleotidase ecto | NM_001204813.1 | 49.7% | 47.5% | (many diffs) |
| 2 | human | 4907 | NT5E | 5'-nucleotidase ecto | NM_002526.4 | 45.4% | 43.4% | (many diffs) |
| 3 | mouse | 23959 | Nt5e | 5' nucleotidase, ecto | NM_011851.4 | 38% | 37.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tccccgagcc gcgcgggcgc ccgcgacgct actcctcgcc ctgggcgcgg 121 tgctgtggcc tgcggctggc gcctgggagc ttacgatttt gcacaccaac gacgtgcaca 181 gccggctgga gcagaccagc gaggactcca gcaagtgcgt caacgccagc cgctgcatgg 241 gtggcgtggc tcggctcttc accaaggttc agcagatccg ccgcgccgaa cccaacgtgc 301 tgctgctgga cgccggcgac cagtaccagg gcactatctg gttcaccgtg tacaagggcg 361 ccgaggtggc gcacttcatg aacgccctgc gctacgatgc catggcactg ggaaatcatg 421 aatttgataa tggtgtggaa ggactgatcg agccactcct caaagaggcc aaatttccaa 481 ttctgagtgc aaacattaaa gcaaaggggc cactagcatc tcaaatatca ggactttatt 541 tgccatataa agttcttcct gttggtgatg aagttgtggg aatcgttGGA TACACTTCCA 601 AAGAAACCCC TTTTCTCTCA AATCCAGGGA CAAATTTAGT GTTTGAAGAT GAAATCACTG 661 CATTACAACC TGAAGTAGAT AAGTTAAAAA CTCTAAATGT GAACAAAATT ATTGCACTGG 721 GACATTCGGG TTTTGAAATG GATAAACTCA TCGCTCAGAA AGTGAGGGGT GTGGACGTCG 781 TGGTGGGAGG ACACTCCAAC ACATTTCTTT ACACAGGTAA TTGTTTCAAA AGGATTGCAT 841 GGGCCAGGAT GTCCAGATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTACAAA GCAGGGAAGA 1021 GGAGGGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt