Construct: ORF TRCN0000473593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016699.1_s317c1
- Derived from:
- ccsbBroadEn_00823
- DNA Barcode:
- ATACGGGACTTATCCAGAGCGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA4 (3441)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 100% | 100% | |
| 2 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 98.2% | 95.7% | (many diffs) |
| 3 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 97.7% | 95.7% | (many diffs) |
| 4 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 96.8% | 93.6% | (many diffs) |
| 5 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 95.9% | 92% | (many diffs) |
| 6 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 94.5% | 88.8% | (many diffs) |
| 7 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 91.1% | 84.1% | (many diffs) |
| 8 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 89.9% | 83.5% | (many diffs) |
| 9 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 89% | 81.4% | (many diffs) |
| 10 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 88.8% | 81.4% | (many diffs) |
| 11 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 88.7% | 81.4% | (many diffs) |
| 12 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 88.3% | 79.8% | (many diffs) |
| 13 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 88% | 80% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 47.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctgtcc ttttctttac tgatggccgt gctggtgctc agctacaaat 121 ccatctgttc tctgggctgt gatctgcctc agacccacag cctgggtaat aggagggcct 181 tgatactcct ggcacaaatg ggaagaatct ctcatttctc ctgcctGAAG GACAGACATG 241 ATTTCGGATT CCCCGAGGAG GAGTTTGATG GCCACCAGTT CCAGAAGGCT CAAGCCATCT 301 CTGTCCTCCA TGAGATGATC CAGCAGACCT TCAATCTCTT CAGCACAGAG GACTCATCTG 361 CTGCTTGGGA ACAGAGCCTC CTAGAAAAAT TTTCCACTGA ACTTTACCAG CAACTGAATG 421 ACCTGGAAGC ATGTGTGATA CAGGAGGTTG GGGTGGAAGA GACTCCCCTG ATGAATGAGG 481 ACTCCATCCT GGCTGTGAGG AAATACTTCC AAAGAATCAC TCTTTATCTA ACAGAGAAGA 541 AATACAGCCC TTGTGCCTGG GAGGTTGTCA GAGCAGAAAT CATGAGATCC CTCTCGTTTT 601 CAACAAACTT GCAAAAAAGA TTAAGGAGGA AGGATTTGCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AATACGGGAC TTATCCAGAG CGCGGACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt