Construct: ORF TRCN0000473604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017622.1_s317c1
- Derived from:
- ccsbBroadEn_07043
- DNA Barcode:
- GCAGTAGTAGTCGAAATACGGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TDGF1 (6997)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473604
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6997 | TDGF1 | teratocarcinoma-derived gro... | NM_003212.4 | 99.4% | 99.4% | 65T>C;342C>T;510A>G |
| 2 | human | 6997 | TDGF1 | teratocarcinoma-derived gro... | NM_001174136.2 | 90.9% | 90.9% | (many diffs) |
| 3 | human | 6998 | TDGF1P3 | teratocarcinoma-derived gro... | NR_002718.2 | 20.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctgcaggaag atggcccgct tctcttacag tgtgatttgg atcatggcca 121 tttctaaagc ctttgaactg ggattagttg ccgggctggg ccatcaggaa tttgctcgtc 181 catctcgggg atacctggcc ttcagagatg acagcatttg gccccaggag gagcctgcaa 241 ttcggcctcg gtcttcccag cgtgtgccgc ccatggggat acagcacagt aaggagctaa 301 acagaacctg ctgcctgaat gggggaacct gcatgctggg gtccttttgt gcctgccctc 361 cctccTTCTA CGGACGGAAC TGTGAGCACG ATGTGCGCAA AGAGAATTGT GGGTCTGTGC 421 CCCATGACAC CTGGCTGCCC AAGAAGTGTT CCCTGTGTAA ATGCTGGCAC GGTCAGCTCC 481 GCTGCTTTCC TCAGGCATTT CTACCCGGCT GTGATGGCCT TGTGATGGAT GAGCACCTCG 541 TGGCTTCCAG GACTCCAGAA CTACCACCGT CTGCGCGTAC TACCACTTTT ATGCTAGTTG 601 GCATCTGCCT TTCTATACAA AGCTACTATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCAGT 781 AGTAGTCGAA ATACGGGCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt