Construct: ORF TRCN0000473608
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005546.1_s317c1
- Derived from:
- ccsbBroadEn_04204
- DNA Barcode:
- TGAAGAACCAATTTCGAATACATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFD (80310)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473608
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80310 | PDGFD | platelet derived growth fac... | NM_033135.4 | 100% | 100% | |
| 2 | human | 80310 | PDGFD | platelet derived growth fac... | NM_025208.5 | 98.3% | 98.3% | 124_141del |
| 3 | mouse | 71785 | Pdgfd | platelet-derived growth fac... | XM_006509885.3 | 85.8% | 84.8% | (many diffs) |
| 4 | mouse | 71785 | Pdgfd | platelet-derived growth fac... | NM_027924.2 | 84.4% | 83.5% | (many diffs) |
| 5 | mouse | 71785 | Pdgfd | platelet-derived growth fac... | XM_006509886.3 | 58.5% | 55.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1158
- ORF length:
- 1092
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ccggctcatc tttgtctaca ctctaatctg cgcaaacttt tgcagctgtc 121 gggacacttc tgcaaccccg cagagcgcat ccatcaaagc tttgcgcaac gccaacctca 181 ggcgagatga cttgtaccga agagatgaga ccatccaggt gaaaggaaac ggctacgtgc 241 agagtcctag attcccgaac agctacccca ggaacctgct cctgacatgg cggcttcact 301 ctcaggagaa tacacggata cagctagtgt ttgacaatca gtttggatta gaggaagcag 361 aaaatgatat ctgtaggtat gattttgtgg aagttgaaga tatatccgaa accagtacca 421 ttattagagg acgatggtgt ggacacaagg aagttcctcc aaggataaaa tcaagaacga 481 accaaattaa aatcacattc aagtccgatg actactttgt ggctaaacct ggattcaaga 541 tttattattc tttgctggaa gatttccaac ccgcagcagc ttcagagacc aactgggaat 601 ctgtcacaag ctctatttca ggggtaTCCT ATAACTCTCC ATCAGTAACG GATCCCACTC 661 TGATTGCGGA TGCTCTGGAC AAAAAAATTG CAGAATTTGA TACAGTGGAA GATCTGCTCA 721 AGTACTTCAA TCCAGAGTCA TGGCAAGAAG ATCTTGAGAA TATGTATCTG GACACCCCTC 781 GGTATCGAGG CAGGTCATAC CATGACCGGA AGTCAAAAGT TGACCTGGAT AGGCTCAATG 841 ATGATGCCAA GCGTTACAGT TGCACTCCCA GGAATTACTC GGTCAATATA AGAGAAGAGC 901 TGAAGTTGGC CAATGTGGTC TTCTTTCCAC GTTGCCTCCT CGTGCAGCGC TGTGGAGGAA 961 ATTGTGGCTG TGGAACTGTC AACTGGAGGT CCTGCACATG CAATTCAGGG AAAACCGTGA 1021 AAAAGTATCA TGAGGTATTA CAGTTTGAGC CTGGCCACAT CAAGAGGAGG GGTAGAGCTA 1081 AGACCATGGC TCTAGTTGAC ATCCAGTTGG ATCACCATGA ACGATGTGAT TGTATCTGCA 1141 GCTCAAGACC ACCTCGATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGAAGAA CCAATTTCGA 1321 ATACATAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt