Construct: ORF TRCN0000473615
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011925.1_s317c1
- Derived from:
- ccsbBroadEn_06406
- DNA Barcode:
- TGGGTACTTTGGGTACTGAGTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPA1A (3303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473615
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3304 | HSPA1B | heat shock protein family A... | NM_005346.6 | 99.8% | 100% | 222T>C;1053G>A;1860C>G |
| 2 | human | 3303 | HSPA1A | heat shock protein family A... | NM_005345.6 | 99.7% | 100% | (many diffs) |
| 3 | human | 3305 | HSPA1L | heat shock protein family A... | NM_005527.4 | 80.9% | 88.1% | (many diffs) |
| 4 | mouse | 193740 | Hspa1a | heat shock protein 1A | NM_010479.2 | 91.5% | 95.3% | (many diffs) |
| 5 | mouse | 15511 | Hspa1b | heat shock protein 1B | NM_010478.2 | 91.4% | 95.1% | (many diffs) |
| 6 | mouse | 15482 | Hspa1l | heat shock protein 1-like | NM_013558.2 | 81.9% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1989
- ORF length:
- 1923
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caaagccgcg gcgatcggca tcgacctggg caccacctac tcctgcgtgg 121 gggtgttcca acacggcaag gtggagatca tcgccaacga ccagggcaac cgcaccaccc 181 ccagctacgt ggccttcacg gacaccgagc ggctcatcgg ggatgcggcc aagaaccagg 241 tggcgctgaa cccgcagaac accgtgtttg acgcgaagcg gctgatcggc cgcaagttcg 301 gcgacccggt ggtgcagtcg gacatgaagc actggccttt ccaggtgatc aacgacggag 361 acaagcccaa ggtgcaggtg agctacaagg gggagaccaa ggcattctac cccgaggaga 421 tctcgtccat ggtgctgacc aagatgaagg agatcgccga ggcgtacctg ggctacccgg 481 tgaccaacgc ggtgatcacc gtgccggcct acttcaacga ctcgcagcgc caggccacca 541 aggatgcggg tgtgatcgcg gggctcaacg tgctgcggat catcaacgag cccacggccg 601 ccgccatcgc ctacggcctg gacagaacgg gcaaggggga gcgcaacgtg ctcatctttg 661 acctgggcgg gggcaccttc gacgtgtcca tcctgacgat cgacgacggc atcttcgagg 721 tgaaggccac ggccggggac acccacctgg gtggggagga ctttgacaac aggctggtga 781 accacttcgt ggaggagttc aagagaaaac acaagaagga catcagccag aacaagcgag 841 ccgtgaggcg gctgcgcacc gcctgcgaga gggccaagag gaccctgtcg tccagcaccc 901 aggccagcct ggagatcgac tccctgtttg agggcatcga cttctacacg tccatcacca 961 gggcgaggtt cgaggagctg tgctccgacc tgttccgaag caccctggag cccgtggaga 1021 aggctctgcg cgacgccaag ctggacaagg cccagattca cgacctggtc ctggtcgggg 1081 gctccacccg catccccaag gtgcagaagc tgctgcaaga cttcttcaac gggcgcgacc 1141 tgaacaagag catcaacccc gacgaggctg tggcctacgg ggcggcggtg caggcggcca 1201 tcctgatggg ggacaagtcc gagaacgtgc aggacctgct gctgctggac gtggctcccc 1261 tgtcgctggg gctggagacg gccggaggcg tgatgactgc cctgatcaag cgcaactcca 1321 ccatccccac caagcagacg cagatcttca ccacctactc cgacaaccaa cccggggtgc 1381 tgatccaggt gtacgagggc gagagggcca tgacgaaaga caacaatctg ttggggcgct 1441 tcgagctgag cggcatccct ccggccccca ggggcgtgcc ccagatcgag gtgaccttcg 1501 acatcgatgc caacggcatc ctgaacgtca cggccacgga caagagcacc ggcaaggcca 1561 acaagatcac catcaCCAAC GACAAGGGCC GCCTGAGCAA GGAGGAGATC GAGCGCATGG 1621 TGCAGGAGGC GGAGAAGTAC AAAGCGGAGG ACGAGGTGCA GCGCGAGAGG GTGTCAGCCA 1681 AGAACGCCCT GGAGTCCTAC GCCTTCAACA TGAAGAGCGC CGTGGAGGAT GAGGGGCTCA 1741 AGGGCAAGAT CAGCGAGGCG GACAAGAAGA AGGTTCTGGA CAAGTGTCAA GAGGTCATCT 1801 CGTGGCTGGA CGCCAACACC TTGGCCGAGA AGGACGAGTT TGAGCACAAG AGGAAGGAGC 1861 TGGAGCAGGT GTGTAACCCC ATCATCAGCG GACTGTACCA GGGTGCCGGT GGTCCCGGGC 1921 CTGGGGGCTT CGGGGCTCAG GGTCCCAAGG GAGGGTCTGG GTCAGGCCCT ACCATTGAGG 1981 AGGTGGATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2041 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2101 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGGGTA CTTTGGGTAC TGAGTGGAAC 2161 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt