Construct: ORF TRCN0000473619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007505.1_s317c1
- Derived from:
- ccsbBroadEn_00198
- DNA Barcode:
- CCCACGATTTGAACTATATTAGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CA1 (759)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 759 | CA1 | carbonic anhydrase 1 | NM_001128829.4 | 100% | 100% | |
| 2 | human | 759 | CA1 | carbonic anhydrase 1 | NM_001128830.4 | 100% | 100% | |
| 3 | human | 759 | CA1 | carbonic anhydrase 1 | NM_001128831.4 | 100% | 100% | |
| 4 | human | 759 | CA1 | carbonic anhydrase 1 | NM_001164830.2 | 100% | 100% | |
| 5 | human | 759 | CA1 | carbonic anhydrase 1 | NM_001738.5 | 100% | 100% | |
| 6 | human | 759 | CA1 | carbonic anhydrase 1 | NM_001291967.2 | 74.7% | 74.7% | 36_37ins198 |
| 7 | human | 759 | CA1 | carbonic anhydrase 1 | NM_001291968.2 | 56.1% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aagtccagac tggggatatg atgacaaaaa tggtcctgaa caatggagca 121 agctgtatcc cattgccaat ggaaataacc agtcccctgt tgatattaaa accagtgaaa 181 ccaaacatga cacctctctg aaacctatta gtgtctccta caacccagcc acagccaaag 241 aaattatcaa tgtggggcat tccttccatg taaattttga ggacaacgat aaccgatcag 301 tgctgaaagg tggtcctttc tcTGACAGCT ACAGGCTCTT TCAGTTCCAT TTTCACTGGG 361 GCAGTACAAA TGAGCATGGT TCAGAACATA CAGTGGATGG AGTCAAATAT TCTGCCGAGC 421 TTCACGTAGC TCACTGGAAT TCTGCAAAGT ACTCCAGCCT TGCTGAAGCT GCCTCAAAGG 481 CTGATGGTTT GGCAGTTATT GGTGTTTTGA TGAAGGTTGG TGAGGCCAAC CCAAAGCTGC 541 AGAAAGTACT TGATGCCCTC CAAGCAATTA AAACCAAGGG CAAACGAGCC CCATTCACAA 601 ATTTTGACCC CTCTACTCTC CTTCCTTCAT CCCTGGATTT CTGGACCTAC CCTGGCTCTC 661 TGACTCATCC TCCTCTTTAT GAGAGTGTAA CTTGGATCAT CTGTAAGGAG AGCATCAGTG 721 TCAGCTCAGA GCAGCTGGCA CAATTCCGCA GCCTTCTATC AAATGTTGAA GGTGATAACG 781 CTGTCCCCAT GCAGCACAAC AACCGCCCAA CCCAACCTCT GAAGGGCAGA ACAGTGAGAG 841 CTTCATTTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCCACG ATTTGAACTA TATTAGTAAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt