Construct: ORF TRCN0000473620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001532.1_s317c1
- Derived from:
- ccsbBroadEn_13393
- DNA Barcode:
- CCCACGGTAGCCCGTACTGAACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM153B (202134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473620
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 202134 | FAM153B | family with sequence simila... | NM_001265615.1 | 80.9% | 79% | (many diffs) |
| 2 | human | 285596 | FAM153A | family with sequence simila... | NM_173663.4 | 80.1% | 77% | (many diffs) |
| 3 | human | 285596 | FAM153A | family with sequence simila... | XM_006714848.3 | 80.1% | 77% | (many diffs) |
| 4 | human | 285596 | FAM153A | family with sequence simila... | XM_006714849.3 | 80.1% | 77% | (many diffs) |
| 5 | human | 285596 | FAM153A | family with sequence simila... | XM_011534521.3 | 80.1% | 77% | (many diffs) |
| 6 | human | 285596 | FAM153A | family with sequence simila... | XM_011534522.2 | 80.1% | 77% | (many diffs) |
| 7 | human | 285596 | FAM153A | family with sequence simila... | XM_011534523.2 | 80.1% | 77% | (many diffs) |
| 8 | human | 285596 | FAM153A | family with sequence simila... | XM_017009366.1 | 80.1% | 77% | (many diffs) |
| 9 | human | 285596 | FAM153A | family with sequence simila... | XM_017009367.1 | 80.1% | 77% | (many diffs) |
| 10 | human | 285596 | FAM153A | family with sequence simila... | XM_017009369.1 | 80.1% | 77% | (many diffs) |
| 11 | human | 285596 | FAM153A | family with sequence simila... | XM_017009370.1 | 80.1% | 77% | (many diffs) |
| 12 | human | 285596 | FAM153A | family with sequence simila... | XM_017009363.1 | 74.4% | 71.5% | (many diffs) |
| 13 | human | 285596 | FAM153A | family with sequence simila... | XM_017009364.1 | 74.4% | 71.5% | (many diffs) |
| 14 | human | 285596 | FAM153A | family with sequence simila... | XM_017009365.1 | 73.8% | 70.9% | (many diffs) |
| 15 | human | 285596 | FAM153A | family with sequence simila... | XM_017009362.1 | 73.3% | 70.5% | (many diffs) |
| 16 | human | 285596 | FAM153A | family with sequence simila... | XM_005265889.3 | 64.3% | 61.7% | (many diffs) |
| 17 | human | 285596 | FAM153A | family with sequence simila... | XM_017009352.1 | 64.3% | 61.7% | (many diffs) |
| 18 | human | 285596 | FAM153A | family with sequence simila... | XM_017009353.1 | 64.3% | 61.7% | (many diffs) |
| 19 | human | 285596 | FAM153A | family with sequence simila... | XM_017009354.1 | 64.3% | 61.7% | (many diffs) |
| 20 | human | 285596 | FAM153A | family with sequence simila... | XM_017009355.1 | 64.3% | 61.7% | (many diffs) |
| 21 | human | 285596 | FAM153A | family with sequence simila... | XM_017009356.1 | 64.3% | 61.7% | (many diffs) |
| 22 | human | 285596 | FAM153A | family with sequence simila... | XM_017009357.1 | 64.3% | 61.7% | (many diffs) |
| 23 | human | 285596 | FAM153A | family with sequence simila... | XM_017009358.1 | 64.3% | 61.7% | (many diffs) |
| 24 | human | 285596 | FAM153A | family with sequence simila... | XM_017009359.1 | 64.3% | 61.7% | (many diffs) |
| 25 | human | 285596 | FAM153A | family with sequence simila... | XM_017009360.1 | 64.3% | 61.7% | (many diffs) |
| 26 | human | 285596 | FAM153A | family with sequence simila... | XM_017009361.1 | 64.3% | 61.7% | (many diffs) |
| 27 | human | 285596 | FAM153A | family with sequence simila... | XM_011534520.2 | 63.8% | 61.2% | (many diffs) |
| 28 | human | 285596 | FAM153A | family with sequence simila... | XM_011534528.2 | 37.3% | 28.6% | (many diffs) |
| 29 | human | 285596 | FAM153A | family with sequence simila... | NR_146227.1 | 16% | (many diffs) | |
| 30 | human | 285596 | FAM153A | family with sequence simila... | NR_146226.1 | 15.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggacaaagac acagagagag acattgagat gaaacggcaa ctacggcgac 121 tacgggagct ccacctatac agcacatgga agaagtacca agaggcgatg aagacatcct 181 tgggagttcc acaatgtgag cgtgacgaag gctccttggg caagccattg tgtccacccg 241 agatactctc ggagacgttg ccaggctctg tgaagaaaag ggtatgcttt ccatcagaag 301 atcatctaga ggagtttata gcagaacatc tccctgaagc atccaatcag agtctcctca 361 ctgttgccca tgcagacaca ggcatccaaa ccaacggtga ccTGGAAGAC CTGGAGGAGC 421 ATGGGCCAGG GCAGACAGTC TCTGAGGAAG CCACAGAAGT TCACATGATG GAGGGGGACC 481 CAGACACACT GGCCGAACTT CTGATCAGGG ATGTACTTCA GGAGCTGTCC AGTTACAACG 541 GCGAGGAGGA GGACCCAGAG GAGGTGAAGA CATCCTTGGG AGTTCCACAA CGTGGTGACC 601 TGGAAGACCT GGAGGAGCAT GTGCCAGGGC AGACAGTCTC TGAGGAAGCC ACAGGGGTTC 661 ACATGATGCA GGTGGACCCA GCCACGCCGG CAAAGAGTGA CCTGGAAGAC CTGGAGGAGC 721 ATGTGCCAGG GCAGACAGTC TCTGAGGAAG CCACAGGGGT TCACATGATG CAGGTGGACC 781 CAGCCACACT GGCAAAGCGT ACGTATTCTG GGATCATCTC TTTGTTTAGG TGTGAAATCT 841 TAGTGTTGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCCACG GTAGCCCGTA CTGAACAAAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt