Construct: ORF TRCN0000473630
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018190.1_s317c1
- Derived from:
- ccsbBroadEn_11173
- DNA Barcode:
- GGATCTACCGCTCGGTTCCTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBP (6908)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473630
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6908 | TBP | TATA-box binding protein | NM_003194.5 | 84.9% | 84.9% | 171_323del |
| 2 | human | 6908 | TBP | TATA-box binding protein | NM_001172085.1 | 79% | 79% | 0_1ins60;111_263del |
| 3 | mouse | 21374 | Tbp | TATA box binding protein | NM_013684.3 | 82.4% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 70
- ORF end:
- 934
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 nttggcacca tggatcagaa caacagcctg ccaccttacg ctcagggctt ggcctcccct 121 cagggtgcca tgactcccgg aatccctatc tttagtccaa tgatgcctta tggcactgga 181 ctgaccccac agcctattca gaacaccaat agtctgtcta ttttggaaga gcaacaaagg 241 caggcaacac agggaacctc aggccaggca ccacagctct tccactcaca gactctcaca 301 actgcaccct tgccgggcac cactccactg tatccctccc ccatgactcc catgaccccc 361 atcactcctg ccacgccagc ttcggagagt tctgggattg taccgcagct gcaaaatatt 421 gtatccacag tgaatcttgg ttgtaaactt gacctaaaga ccattgcact tcgtgcccga 481 aacgccgaat ataatcccaa gcggtttgct gcggtaatca tgaggataag agagccacga 541 accacggcac tgattttcag ttctgggaaa atggtgtgca caggagccaa gagtgaagaa 601 cagtccagac tggcagcaag aaaatatgct agagttgtac agaagttggg ttttccagct 661 aagttcttgg acttcaagat tcagaatatG GTGGGGAGCT GTGATGTGAA GTTTCCTATA 721 AGGTTAGAAG GCCTTGTGCT CACCCACCAA CAATTTAGTA GTTATGAGCC AGAGTTATTT 781 CCTGGTTTAA TCTACAGAAT GATCAAACCC AGAATTGTTC TCCTTATTTT TGTTTCTGGA 841 AAAGTTGTAT TAACAGGTGC TAAAGTCAGA GCAGAAATTT ATGAAGCATT TGAAAACATC 901 TACCCTATTC TAAAGGGATT CAGGAAGACG ACGTTGCCAA CTTTCTTGTA CAAAGTGGTT 961 GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT 1021 AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG 1081 GATCTACCGC TCGGTTCCTA ACCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 1141 tttgtgaaag att