Construct: ORF TRCN0000473632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009985.1_s317c1
- Derived from:
- ccsbBroadEn_02005
- DNA Barcode:
- AGCCACTTTTAAAGGACCTTTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM21 (8747)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8747 | ADAM21 | ADAM metallopeptidase domai... | NM_003813.4 | 99.5% | 99.4% | (many diffs) |
| 2 | human | 145241 | ADAM21P1 | ADAM metallopeptidase domai... | NR_003951.1 | 81% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2235
- ORF length:
- 2166
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagtggat gggaccctcg tgtacatcag ggtcactctt ctgctgctct 121 ggcttggggt atttttgtct atttccggct actgtcaggc tgggccctcc cagcatttca 181 cttccccgga agtggtgatc cccttgaagg tgatcagcag gggcagaagt gcaaaggctc 241 ctggatggct ctcctatagt ctgcggtttg ggggccagaa acacgttgtt catatgaggg 301 tcaagaagct cttagtttct agacacctcc cagtgttcac ctacacagat gagcgtgcac 361 tcctggagga tcagctcttc atcccagatg actgttacta tcatggttac gtggagggtg 421 cccctgagtc tctggttgtg ttcagtgctt gttttggggg ctttcgagga gtattaaaaa 481 taagtggcct cacttatgaa attgaaccca tcaggcactc tgccacattt gaacacctgg 541 tttacaaagt aaacagtaat gagacacaat tcccagctat gagatgtggc ttaacagaga 601 aggaagtagc acgccaacag ttggaatttg aagaggctga gaactcagct ctggaaccaa 661 aatctgctgg tgactggtgg actcatgcat ggtttctgga gctagttgtt gtggtgaacc 721 atgatttctt catttactct caaagcaaca tctcaaaggt gcaagaggat gtatttcttg 781 ttgtcaacat agtggattcc atgtatcagc agttaggtac ttacataatt ttgattggaa 841 ttgaaatttg gaatcaagga aatgttttcc caatgacaag catagaacag gtcctgaacg 901 atttctctca atggaaacaa atcagtcttt cccagctaca gcatgatgct gcacatatgt 961 tcataaaaaa ttcacttata agtatacttg gcctagccta tgttgcagga atatgtcgtc 1021 cacctattga ttgtggagtt gataattttc aaggagatac ctggtctctt tttgccaaca 1081 ctgtggccca tgagttaggt catacgttgg gtatgcagca tgatgaagaa ttctgttttt 1141 gtggggaaag aggttgcatc atgaatactt ttagagtgcc agcagagaaa ttcaccaatt 1201 gcagttacgc tgattttatg aagaccacct taaaccaggg atcatgtctg cataatcctc 1261 caagattggg ggaaatcttt atgctaaagc gctgtgggaa tggtgtggtt gaaagagaag 1321 agcagtgtga ctgtggatcc gtacagcagt gtgaacaaga cgcctgttgt ctgttgaact 1381 gcactctaag gcctggggct gcctgtgctt ttgggctttg ttgcaaagac tgcaagttca 1441 tgccatcagg ggaactctgt agacaagagg tcaatgaatg tgaccttcca gaatggtgca 1501 atggaacatc tcatcagtgt ccagaagata gatatgtgca ggacgggatc ccctgtagtg 1561 acagtgccta ctgctatcaa aagaggtgta ataaccatga ccagcattgc agggagattt 1621 ttggtaaaga tgcaaaaagt gcatctcaga attgctataa agaaatcaac tctcagggaa 1681 accgttttgg tcactgtggt ataaatggca caacatacct aaaatgtcat atctctgatg 1741 tcttttgtgg gagagttcaa tgtgagaatg tgagagacat tcctcttcTC CAAGATCATT 1801 TTACTTTGCA GCACACTCAT ATCAATGGTG TCACCTGCTG GGGTATTGAC TATCATTTAA 1861 GGATGAACAT ATCTGACATT GGTGAAGTGA AAGATGGTAC TGTGTGTGGC CCAGGAAAGA 1921 TCTGCATCCA TAAGAAGTGT GTCAGTCTGT CTGTCTTGTC ACATGTCTGC CTTCCTGAGA 1981 CCTGCAATAT GAAGGGGATC TGCAATAACA AACATCACTG CCACTGTGGC TATGGGTGGT 2041 CCCCACCCTA CTGCCAGCAC AGAGGCTATG GGGGCAGTAT TGACAGTGGC CCAGCATCTG 2101 CAAAGAGAGG AGTTTTTTTG CCGCTGATTG TGATTCCTTC TTTGTCTGTT TTGACTTTCC 2161 TGTTTACTGT CGGGCTTCTT ATGTATCTAC GACAATGTTC TGGTCCCAAA GAAACTAAGG 2221 CTCATTCATC AGGTTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2281 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2341 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGCCACTTTT AAAGGACCTT 2401 TGTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt