Construct: ORF TRCN0000473638
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013576.1_s317c1
- Derived from:
- ccsbBroadEn_04467
- DNA Barcode:
- CTGTTCTTGGGGTCCTCCGGTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCEAL3 (85012)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473638
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85012 | TCEAL3 | transcription elongation fa... | NM_001006933.1 | 100% | 100% | |
| 2 | human | 85012 | TCEAL3 | transcription elongation fa... | NM_032926.3 | 100% | 100% | |
| 3 | human | 158931 | TCEAL6 | transcription elongation fa... | NM_001367790.1 | 98% | 96.5% | (many diffs) |
| 4 | human | 340543 | TCEAL5 | transcription elongation fa... | NM_001012979.3 | 90.3% | 88.3% | (many diffs) |
| 5 | human | 158931 | TCEAL6 | transcription elongation fa... | NM_001006938.3 | 89.8% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 666
- ORF length:
- 600
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaaaccctac aataaaaatg aaggaaacct ggaaaacgag ggaaagccag 121 aagatgaagt agagcctGAT GATGAAGGAA AGTCAGACGA GGAAGAAAAG CCAGACGTGG 181 AGGGGAAGAC AGAATGCGAG GGAAAGAGAG AGGATGAGGG AGAGCCAGGT GATGAGGGAC 241 AACTGGAAGA TGAGGGAAGC CAGGAAAAGC AGGGCAGGTC CGAAGGTGAG GGCAAGCCAC 301 AAGGCGAGGG CAAGCCAGCC TCCCAGGCAA AGCCAGAGAG CCAGCCGCGG GCCGCCGAAA 361 AGCGCCCGGC TGAAGATTAT GTGCCCCGGA AAGCAAAAAG AAAAACGGAC AGGGGGACGG 421 ACGATTCCCC CAAGGACTCT CAGGAGGACT TACAGGAAAG GCATCTGAGC AGTGAGGAGA 481 TGATGAGAGA ATGTGGAGAT GTGTCAAGGG CTCAAGAGGA GCTAAGGAAA AAACAGAAAA 541 TGGGTGGTTT TCATTGGATG CAAAGAGATG TACAGGATCC ATTCGCCCCA AGGGGACAAC 601 GGGGTGTCAG GGGAGTGAGG GGTGGAGGTA GGGGCCAGAG GGGCTTACAC GATATCCCAT 661 ACCTTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 721 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 781 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGTTCTTG GGGTCCTCCG GTTATACGCG 841 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt