Construct: ORF TRCN0000473666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012282.1_s317c1
- Derived from:
- ccsbBroadEn_04672
- DNA Barcode:
- AGCCGAACGTCTCAAACCCCCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLYWCH2 (114984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114984 | FLYWCH2 | FLYWCH family member 2 | NM_001142499.1 | 100% | 100% | |
| 2 | human | 114984 | FLYWCH2 | FLYWCH family member 2 | NM_001142500.1 | 100% | 100% | |
| 3 | human | 114984 | FLYWCH2 | FLYWCH family member 2 | NM_138439.3 | 100% | 100% | |
| 4 | human | 114984 | FLYWCH2 | FLYWCH family member 2 | XM_005255078.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cctgcccgag cccagcgagc aggagggtga gagtgtgaag gccagccagg 121 agccatcccc caagccaggc acagaagtca tcccggcagc ccccaggaag cccagaaagt 181 tctccaaact ggtcctgctc acagcctcca aagacagcac caaggtggcg ggggccaagc 241 gcaagggtgt gcacTGTGTC ATGTCCCTGG GGGTGCCCGG CCCCGCCACC CTTGCCAAGG 301 CCCTCCTCCA GACCCACCCC GAGGCCCAGC GGGCCATTGA GGCAGCCCCT CAGGAGCCTG 361 AGCAGAAACG GAGCAGGCAG GACCCAGGCA CAGACAGAAC AGAAGACAGT GGATTAGCAG 421 CGGGGCCTCC TGAGGCTGCT GGGGAGAACT TTGCCCCCTG CTCTGTGGCG CCCGGCAAGT 481 CCCTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGCCGAACG TCTCAAACCC CCACCACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt