Construct: ORF TRCN0000473667

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004407.4_s317c1
Derived from:
ccsbBroadEn_08709
DNA Barcode:
CCCCCCCTCGTAAGTGGTCATACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCYL3 (57147)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473667
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57147 SCYL3 SCY1 like pseudokinase 3 NM_020423.7 99.8% 99.8% (many diffs)
2 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_017001862.1 99.8% 99.8% (many diffs)
3 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_017001863.1 99.8% 99.8% (many diffs)
4 human 57147 SCYL3 SCY1 like pseudokinase 3 NM_181093.3 92.5% 92.5% (many diffs)
5 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_006711465.1 92.5% 92.5% (many diffs)
6 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_011509801.1 92.5% 92.5% (many diffs)
7 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_017001864.1 79.1% 79.2% (many diffs)
8 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_017001865.1 79.1% 79.2% (many diffs)
9 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_011509802.1 73.4% 73.4% (many diffs)
10 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_011509803.1 73.4% 73.4% (many diffs)
11 human 57147 SCYL3 SCY1 like pseudokinase 3 XR_001737336.2 69.8% (many diffs)
12 human 57147 SCYL3 SCY1 like pseudokinase 3 XR_001737335.2 54.4% (many diffs)
13 human 57147 SCYL3 SCY1 like pseudokinase 3 XM_024448565.1 44.8% 44.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2130
ORF length:
2064
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg atcagagaac agtgctttaa agagctatac actgagagaa ccaccattta
121ccttaccctc tggacttgct gtttatcccg ctgtactgca agatggcaaa tttgcttcag
181tttttgtgta taagagagaa aatgaagaca aggttaataa agctgccaag catttgaaga
241cacttcgtca cccttgcttg ctaagatttt tatcttgtac tgtggaagcg gatggcattc
301atcttgtcac tgagcgagta cagcccctgg aagtggcttt ggaaacattg tcttctgcag
361aggtctgtgc tgggatctat gacatattgc tggctcttat cttccttcat gacagaggac
421acctaacaca caataatgtc tgtttatcat ctgtgtttgt gagtgaagat ggacactgga
481agctaggagg aatggaaact gtttgtaaag tttctcaggc cacaccagag tttctgagga
541gtattcagtc aataagagac ccagcatcta tccctcctga agagatgtct ccagaattca
601caactctccc agagtgtcat ggacatgccc gggatgcctt ttcatttgga acattggtgg
661aaagtttgct cacaatctta aatgaacagg tttcagcgga tgttctctcc agctttcaac
721agaccttgca ctcaactttg ctgaatccca ttccaaaatg tcggccagcg ctctgcacct
781tactatctca tgacttcttc agaaatgatt ttctggaagt tgtgaatttc ttgaaaagtt
841taacattgaa gagtgaagag gagaaaacgg aattctttaa atttctgctg gacagagtca
901gctgcttgtc agaggaattg atagcttcaa ggttggtgcc tcttctgctt aatcagttgg
961tgtttgcaga gccagtggct gttaagagtt ttcttcctta tctgcttggc cccaaaaaag
1021atcatgcgca gggagaaact ccttgcttgc tctcaccagc cctgttccag tcacgggtga
1081tccccgtgct tctccagttg tttgaagttc atgaagagca tgtgcggatg gtgctgctgt
1141ctcacatcga ggcctacgtg gagcacttca ctcaggagca gctgaagaaa gtcatcttgc
1201cacaggtttt gctgggcctg cgtgatacta gtgattccat tgtggcaatt actctgcata
1261gcctagcagt gctggtctct ctgcttggac cagaggtggt tgtgggagga gaacgaacca
1321agatcttcaa acgcactgcc ccaagtttta ctaaaaatac tgacctttct ctagaaggtg
1381atccattttc tcagcctatt aaatttccca taaacggact ctcagatgta aaaaatactt
1441cggaggacag tgaaaacttc ccatcaagtt ctaaaaagtc tgaggagtgg cctgactgga
1501gtgaacctga ggagcctgaa aatcaaactg tcaacataca gatttggcct agagaacctt
1561gtgatgatgt caagtcccag tgcactacct tggatgtgga agagtcatct tgggatgact
1621gcgagcccag cagcttagat actaaagtaa acccaggagg tggaatcact gctacaaaac
1681ctgttacctc agcggagcag aagcctattc ctgctttgct ttcactcact gaagagtcta
1741tgccttggaa atcaagctta ccccaaaaga ttagccttgt acaaaggggg gatgacgcag
1801accaaatcga gccgccaaaa gtgtcatcac aagaaaggcc ccttaaggtt ccatcagaac
1861ttggtttagg agaggaattc accattcaag taaaaaagaa gccagtaaaa gatcctgaga
1921tggattggtt tgctgatatg atcccagaaa ttaagccttc tgctgctttt cttatattac
1981ctgaactgag gacagaaatg gtcccaaaaa aggatgatgt ctccccagtg atgcagtttt
2041cctcaaaatt tgcTGCAGCA GAAATTACTG AGGGAGAGGC TGAAGGCTGG GAAGAAGAAG
2101GGGAGCTGAA CTGGGAAGAT AATAACTGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA
2161TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2221CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCCCC
2281CCTCGTAAGT GGTCATACAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2341tgaaagatt

Download FASTA (ORF) (Full)