Construct: ORF TRCN0000473673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018155.1_s317c1
- Derived from:
- ccsbBroadEn_08636
- DNA Barcode:
- TTTGCCCAGCCCTCCACAGTGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRAP (56246)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473673
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56246 | MRAP | melanocortin 2 receptor acc... | NM_178817.4 | 99.6% | 100% | 132G>T;390C>G |
| 2 | human | 56246 | MRAP | melanocortin 2 receptor acc... | XM_006724028.3 | 99.6% | 100% | 132G>T;390C>G |
| 3 | human | 56246 | MRAP | melanocortin 2 receptor acc... | NM_001285394.2 | 65.5% | 65.6% | 0_1ins177;213C>G |
| 4 | human | 56246 | MRAP | melanocortin 2 receptor acc... | NM_206898.1 | 52.7% | 43.1% | (many diffs) |
| 5 | human | 56246 | MRAP | melanocortin 2 receptor acc... | XM_017028407.1 | 52.7% | 43.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caacgggacc aacgcctctg ccccatacta cagctatgaa tactacctgg 121 actatctgga cctcattccc gtggacgaga agaagctgaa agcccacaaa cattccatcg 181 tgatcgcatt ctgggttagc ctggctgcct tcgtggtgct gctcttcctc atcttgctct 241 acatgtcctg gtccgcctcc ccgcagatga ggaacagccc caagcaccac caaacatgcc 301 cctGGAGTCA CGGCCTCAAC CTCCACCTCT GCATCCAGAA GTGCCTGCCG TGCCACAGGG 361 AACCCCTGGC AACCTCACAG GCTCAGGCGA GCTCAGTGGA GCCAGGGAGC AGAACTGGCC 421 CTGACCAGCC GCTACGACAG GAGAGCTCCT CCACGTTGCC CCTCGGGGGT TTCCAGACCC 481 ACCCCACTCT CCTCTGGGAA CTGACCCTCA ATGGGGGTCC CCTCGTCAGG AGCAAGCCCA 541 GCGAGCCTCC CCCTGGAGAC AGGACCTCTC AATTGCAGAG CTGCCTAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGATTT GCCCAGCCCT CCACAGTGAT TACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t