Construct: ORF TRCN0000473674
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003271.1_s317c1
- Derived from:
- ccsbBroadEn_02310
- DNA Barcode:
- CAGACCGGTTTTTCCCAGCATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARPC4 (10093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473674
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10093 | ARPC4 | actin related protein 2/3 c... | NM_005718.4 | 100% | 100% | |
| 2 | human | 10093 | ARPC4 | actin related protein 2/3 c... | NM_001198780.2 | 89.4% | 89.3% | 1_59delinsAT |
| 3 | human | 10093 | ARPC4 | actin related protein 2/3 c... | NM_001024959.2 | 46.4% | 46.4% | 0_1ins270 |
| 4 | human | 10093 | ARPC4 | actin related protein 2/3 c... | NM_001024960.2 | 46.4% | 46.4% | 0_1ins270 |
| 5 | human | 100526693 | ARPC4-TTLL3 | ARPC4-TTLL3 readthrough | NM_001198793.1 | 24.3% | 19.3% | (many diffs) |
| 6 | mouse | 68089 | Arpc4 | actin related protein 2/3 c... | NM_026552.3 | 92.4% | 100% | (many diffs) |
| 7 | mouse | 68089 | Arpc4 | actin related protein 2/3 c... | NM_001170485.1 | 49.5% | 53.5% | (many diffs) |
| 8 | mouse | 68089 | Arpc4 | actin related protein 2/3 c... | NM_001170486.1 | 43.2% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgccactctc cgcccctacc tgagtgccgt gcgggccaca ttgcaggctg 121 ccctctgcct ggagaacttc tcctcccagg ttgtggaacg acacaacaag ccggaagtgg 181 aagtcaggag tagcaaagag ctccTGTTAC AACCTGTGAC CATCAGCAGG AATGAGAAGG 241 AAAAGGTTCT GATTGAGGGC TCCATCAACT CTGTCCGGGT CAGCATTGCT GTGAAACAGG 301 CTGATGAGAT CGAGAAGATT TTGTGCCACA AGTTCATGCG CTTCATGATG ATGCGAGCAG 361 AGAACTTCTT TATCCTTCGA AGGAAGCCTG TGGAGGGGTA TGATATCAGC TTTCTGATCA 421 CCAACTTCCA CACAGAGCAG ATGTACAAAC ACAAGTTGGT GGACTTTGTG ATCCACTTCA 481 TGGAGGAGAT TGACAAGGAG ATCAGTGAGA TGAAGCTGTC AGTCAATGCC CGTGCCCGCA 541 TTGTGGCTGA AGAGTTCCTT AAGAATTTTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAGAC 721 CGGTTTTTCC CAGCATCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt