Construct: ORF TRCN0000473680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004838.1_s317c1
- Derived from:
- ccsbBroadEn_00435
- DNA Barcode:
- ATTTTGTTGGGGTTAGCCCAGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEFA3 (1668)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1668 | DEFA3 | defensin alpha 3 | NM_005217.4 | 100% | 100% | |
| 2 | human | 728358 | DEFA1B | defensin alpha 1B | NM_001042500.1 | 99.6% | 98.9% | 194C>A |
| 3 | human | 1667 | DEFA1 | defensin alpha 1 | NM_004084.3 | 99.6% | 98.9% | 194C>A |
| 4 | human | 1668 | DEFA3 | defensin alpha 3 | XM_011534741.2 | 93% | 93% | 1_21del |
| 5 | human | 728358 | DEFA1B | defensin alpha 1B | NM_001302265.1 | 92.7% | 92% | 1_21del;215C>A |
| 6 | human | 1667 | DEFA1 | defensin alpha 1 | XM_011534740.3 | 92.7% | 92% | 1_21del;215C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 348
- ORF length:
- 282
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaccctcgcc atCCTTGCTG CCATTCTCCT GGTGGCCCTG CAGGCCCAGG 121 CTGAGCCACT CCAGGCAAGA GCTGATGAGG TTGCTGCAGC CCCGGAGCAG ATTGCAGCGG 181 ACATCCCAGA AGTGGTTGTT TCCCTTGCAT GGGACGAAAG CTTGGCTCCA AAGCATCCAG 241 GCTCAAGGAA AAACATGGAC TGCTATTGCA GAATACCAGC GTGCATTGCA GGAGAACGTC 301 GCTATGGAAC CTGCATCTAC CAGGGAAGAC TCTGGGCATT CTGCTGCTGC CCAACTTTCT 361 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 421 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 481 GTGGAAAGGA CGAATTTTGT TGGGGTTAGC CCAGGATACG CGTTAAGTCg acaatcaacc 541 tctggattac aaaatttgtg aaagatt