Construct: ORF TRCN0000473687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001903.1_s317c1
- Derived from:
- ccsbBroadEn_11977
- DNA Barcode:
- TTCGCCTTTTCAAAATTCGCCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL6IP4 (51329)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NM_001278380.1 | 96.4% | 96.4% | 126_149del |
| 2 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NM_001278379.1 | 77.3% | 77.3% | 1_192del |
| 3 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NM_016638.3 | 54.2% | 54.2% | 1_552del |
| 4 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NM_001002251.2 | 52.7% | 52.7% | 1_552del;679_711del |
| 5 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NM_018694.3 | 51.7% | 51.7% | 1_552del;678_734del |
| 6 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NM_001002252.2 | 51.5% | 49% | (many diffs) |
| 7 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NM_001278378.1 | 41.1% | 41.1% | 1_552del;679_711del;923_924ins144 |
| 8 | human | 51329 | ARL6IP4 | ADP ribosylation factor lik... | NR_103512.1 | 29.3% | (many diffs) | |
| 9 | mouse | 65105 | Arl6ip4 | ADP-ribosylation factor-lik... | NM_144509.2 | 78.9% | 79.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcacgtcggc tcccgcaagc gctcgaggag tcgcagccgg tcccggggac 121 gggggtcgga aaagagaaag aagaagagca ggaaagacac ctcgaggaac tgctcggcct 181 ccacatccca agagagaagc aagcagaagg cccggaggag aacaagatcc agctcctcct 241 cctcttcttc cagttcttct agctcctctt cttcctcctc gtcctcctcc tcttcctcca 301 gtgatggccg gaagaagcgg gggaagtaca aggacaagag gaggaagaag aagaagaaga 361 ggaagaagct gaagaagaag ggcaaggaga aggcggaagc acagcaggtg gaggctctgc 421 cgggcccctc gctggaccag tggcaccgat cagctgggga ggaagaggat ggcccagtcc 481 tgacggatga gcagaagtcc cGAATCCAGG CCATGAAGCC CATGACCAAG GAGGAGTGGG 541 ATGCCCGGCA GAGCATCATC CGCAAGGTGG TGGACCCTGA GACGGGGCGC ACCAGGCTTA 601 TTAAGGGAGA TGGCGAGGTC CTAGAGGAAA TCGTAACCAA AGAACGACAC AGAGAGATCA 661 ACAAGCAAGC CACCCGAGGG GACTGCCTGG CCTTCCAGAT GCGAGCTGGG TTGCTTCCCT 721 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGATTCGC CTTTTCAAAA TTCGCCAATA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt