Construct: ORF TRCN0000473688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012643.1_s317c1
- Derived from:
- ccsbBroadEn_07607
- DNA Barcode:
- AAACTCCCCTATCGTGTACGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1D (10438)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473688
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10438 | C1D | C1D nuclear receptor corepr... | NM_001190263.2 | 99.7% | 100% | 417T>C |
| 2 | human | 10438 | C1D | C1D nuclear receptor corepr... | NM_001190265.2 | 99.7% | 100% | 417T>C |
| 3 | human | 10438 | C1D | C1D nuclear receptor corepr... | NM_006333.4 | 99.7% | 100% | 417T>C |
| 4 | human | 10438 | C1D | C1D nuclear receptor corepr... | NM_173177.3 | 99.7% | 100% | 417T>C |
| 5 | mouse | 57316 | C1d | C1D nuclear receptor co-rep... | NM_001330649.1 | 87% | 90% | (many diffs) |
| 6 | mouse | 57316 | C1d | C1D nuclear receptor co-rep... | NM_020558.4 | 87% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC AGGTGAAGAA ATTAATGAAG ACTATCCAGT AGAAATTCAC GAGTATTTGT 121 CAGCGTTTGA GAATTCCATT GGTGCTGTGG ATGAGATGCT GAAGACCATG ATGTCTGTTT 181 CTAGAAATGA GTTGTTGCAG AAGTTGGATC CACTTGAACA AGCAAAAGTG GATTTGGTTT 241 CTGCATACAC ATTAAATTCA ATGTTTTGGG TTTATTTGGC AACCCAAGGA GTTAATCCTA 301 AGGAACATCC AGTAAAACAG GAATTGGAAA GAATCAGAGT ATATATGAAC AGAGTCAAGG 361 AAATAACAGA CAAGAAAAAG GCTGGCAAGC TGGACAGAGG TGCAGCTTCA AGATTTGTAA 421 AAAATGCCCT CTGGGAACCA AAATCGAAAA ATGCATCAAA AGTTGCCAAT AAAGGAAAAA 481 GCAAAAGTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAACTC CCCTATCGTG TACGCCTTAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt