Construct: ORF TRCN0000473695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010241.1_s317c1
- Derived from:
- ccsbBroadEn_03353
- DNA Barcode:
- GAGACGCCGTACGACATTTTTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIL1 (51645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473695
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51645 | PPIL1 | peptidylprolyl isomerase li... | NM_016059.5 | 100% | 100% | |
| 2 | mouse | 68816 | Ppil1 | peptidylprolyl isomerase (c... | NM_026845.4 | 88.1% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcaattccc ccagattcct ggcagccacc caacgtttac ttggagacca 121 gcatgggaat cattgtgctg gagctgtact ggaagcatgc tccaaagacc tgtaagaact 181 ttgctgagtt ggcTCGTCGA GGTTACTACA ATGGCACAAA ATTCCACAGA ATTATCAAAG 241 ACTTCATGAT CCAAGGAGGT GACCCAACAG GGACAGGTCG AGGTGGTGCA TCTATCTATG 301 GCAAACAGTT TGAAGATGAA CTTCATCCAG ACTTGAAATT CACGGGGGCT GGAATTCTCG 361 CAATGGCCAA TGCGGGGCCA GATACCAATG GCAGCCAGTT CTTTGTGACC CTCGCCCCCA 421 CCCAGTGGCT TGACGGCAAA CACACCATTT TTGGCCGAGT GTGTCAGGGC ATAGGAATGG 481 TGAATCGCGT GGGAATGGTA GAAACAAACT CCCAGGACCG CCCTGTGGAC GACGTGAAGA 541 TCATTAAGGC ATACCCTTCT GGGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG AGACGCCGTA 721 CGACATTTTT AAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att