Construct: ORF TRCN0000473704
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010919.1_s317c1
- Derived from:
- ccsbBroadEn_13221
- DNA Barcode:
- TCGTAGGCGATTCCTCTCAGTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OVOS2 (144203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473704
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 728715 | LOC728715 | ovostatin homolog 2 | NR_144634.1 | 25.1% | (many diffs) | |
| 2 | human | 144203 | OVOS2 | alpha-2-macroglobulin like ... | NR_153414.1 | 4.6% | (many diffs) | |
| 3 | human | 408186 | OVOS | alpha-2-macroglobulin like ... | NR_153413.1 | 4.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 223
- ORF end:
- 508
- ORF length:
- 285
- Sequence:
-
1 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC 61 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT 121 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG 181 GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGGTGGT TATAGATGTA 241 AAAATGCTAT CAGGATTTAC TCCAACCATG TCATCCATTG AAGAGCTTGA AAACAAGGGC 301 CAAGTGATGA AGACTGAAGT CAAGAATGAC CATGTTCTTT TCTACTTGGA AAATGTTTTT 361 GGTCGAGCAG ACAGTTTCAC TTTTTCTGTT GAGCAGAGCA ACCTTGTGTT CAACATTCAG 421 CCAGCCCCAG GCATGGTCTA CGATTACTAC GAAAAAGAAG AATATGCCCT AGCTTTTTAC 481 CACATCAACA GTAGTTCAGT TTCCGAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC 541 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 601 CAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCGTAGG 661 CGATTCCTCT CAGTATGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 721 aaagatt