Construct: ORF TRCN0000473706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018330.1_s317c1
- Derived from:
- ccsbBroadEn_04069
- DNA Barcode:
- CAAGATGCGAGAAACCGAATGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRCC3 (79184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79184 | BRCC3 | BRCA1/BRCA2-containing comp... | NM_024332.3 | 100% | 100% | |
| 2 | human | 79184 | BRCC3 | BRCA1/BRCA2-containing comp... | XM_005274751.4 | 99.6% | 99.6% | 139_141delAGT |
| 3 | human | 79184 | BRCC3 | BRCA1/BRCA2-containing comp... | NM_001018055.2 | 92% | 92% | 548_549ins75 |
| 4 | human | 79184 | BRCC3 | BRCA1/BRCA2-containing comp... | XM_017029838.1 | 92% | 92% | 548_549ins75 |
| 5 | human | 79184 | BRCC3 | BRCA1/BRCA2-containing comp... | NM_001242640.1 | 91.7% | 91.7% | 139_141delAGT;551_552ins75 |
| 6 | mouse | 210766 | Brcc3 | BRCA1/BRCA2-containing comp... | NM_001166457.1 | 84.3% | 89.5% | (many diffs) |
| 7 | mouse | 210766 | Brcc3 | BRCA1/BRCA2-containing comp... | NM_001166459.1 | 84.3% | 89.5% | (many diffs) |
| 8 | mouse | 210766 | Brcc3 | BRCA1/BRCA2-containing comp... | NM_145956.4 | 84.3% | 89.5% | (many diffs) |
| 9 | mouse | 210766 | Brcc3 | BRCA1/BRCA2-containing comp... | XM_011247554.2 | 84.3% | 89.5% | (many diffs) |
| 10 | mouse | 368203 | Gm5136 | predicted gene 5136 | NM_203660.2 | 77.4% | 79.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtgcaggtg gtgcaggcgg tgcaggcggt tcatctcgag tctgacgctt 121 tcctcgtttg tctcaaccac gctctgagca cagagaagga ggaagtaatg gggctgtgca 181 taggggagtt gaacgatgat acaaggagtg actccaaatt tgcatatact ggaactgaaa 241 tgcgcacagt tgctgaaaag gttgatgccg tcagaattgt tcacattcat tctgtcatca 301 tcttacgacg ttctgataag aggaaggacc gagtagaaat ttctccagag cagctgtctg 361 cagcttcaac agaggcagag aggttggctg aactgacagg ccgccccatg agagttgtgg 421 gctggtatca ttcccatcct catataactg tttggccttc acatgttgat gttcGCACAC 481 AAGCCATGTA CCAGATGATG GATCAAGGCT TTGTAGGACT TATTTTTTCC TGTTTCATAG 541 AAGATAAGAA CACAAAGACT GGCCGGGTAC TCTACACTTG CTTCCAATCC ATACAGGCCC 601 AAAAGAGTTC AGAGTCCCTT CATGGTCCAC GAGACTTCTG GAGCTCCAGC CAGCACATCT 661 CCATTGAGGG CCAGAAGGAA GAGGAAAGGT ATGAGAGAAT CGAAATCCCA ATCCATATTG 721 TACCTCATGT CACTATCGGG AAAGTGTGCC TTGAATCAGC AGTAGAGCTG CCCAAGATCC 781 TGTGCCAGGA GGAGCAGGAT GCGTATAGGA GGATCCACAG CCTTACACAT CTGGACTCAG 841 TAACCAAGAT CCATAATGGC TCAGTGTTTA CCAAGAATCT GTGCAGTCAG ATGTCGGCAG 901 TCAGCGGGCC TCTCCTACAG TGGTTGGAGG ACAGACTGGA GCAAAACCAA CAGCATTTGC 961 AGGAATTACA ACAAGAAAAG GAAGAGCTTA TGCAAGAACT TTCTTCTCTA GAATACCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAC AAGATGCGAG AAACCGAATG AAAACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att