Construct: ORF TRCN0000473713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000520.1_s317c1
- Derived from:
- ccsbBroadEn_12899
- DNA Barcode:
- GCAAAGACACGTAGACGATAAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM83A (84985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84985 | FAM83A | family with sequence simila... | NM_001288587.3 | 99.8% | 99.6% | 541G>A |
| 2 | human | 84985 | FAM83A | family with sequence simila... | NM_207006.2 | 79.5% | 78.2% | (many diffs) |
| 3 | human | 84985 | FAM83A | family with sequence simila... | XM_005251087.3 | 77.5% | 76.7% | (many diffs) |
| 4 | human | 84985 | FAM83A | family with sequence simila... | NM_032899.6 | 67.5% | 66.8% | (many diffs) |
| 5 | human | 84985 | FAM83A | family with sequence simila... | NM_001321630.2 | 60% | 58.1% | (many diffs) |
| 6 | human | 84985 | FAM83A | family with sequence simila... | XM_011517338.2 | 57.9% | 57.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 948
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccggtcaagg cacctgggca aaatccggaa gcgtctggaa gatgtcaaga 121 gccagtgggt ccggccagcc agggctgact ttagtgacaa cgagagtgcc cggctggcca 181 cggacgccct cttggatggg ggttctgaag cctactggcg ggtgctcagc caggaaggcg 241 aggtggactt cttgtcctcg gtggaggccc agtacatcca ggcccaggcc agggagcccc 301 cgtgtccccc agacaccctg ggaggggcgg aagcaggccc taagggactg gactccagct 361 ccctacagtc cggcacctac ttccctgtgg cctcagaggg cagcgagccg gccctactgc 421 acagctgggc ctcagctgag aagccctacc tgaaggaaaa atccagcgcc actgtgtact 481 tccagaccgt caagcacaac aacatcagag accTCGTCCG CCGCTGCATC ACCCGGACTA 541 GCCAGAACAT TTCCATCCGG AGTGTGGAAG GAGAGATATA CTGTGCCAAG TCAGGCAGGA 601 AATTCACTGG CCAAATCCGG GAGAAGTTCA TCATCTCGGA CTGGAGATTT GTCCTGTCTG 661 GATCTTACAG CTTCACCTGG CTCTGCGGAC ACGTGCACCG GAACATCCTC TCCAAGTTCA 721 CAGGCCAGGC GGTGGAGCTG TTTGACGAGG AGTTCCGCCA CCTCTACGCC TCCTCCAAGC 781 CTGTGATGGG CCTGAAGTCC CCGCGGCTGG TCGCCCCCGT CCCGCCCGGA GCAGCCCCGG 841 CCAATGGCCG CCTTAGCAGC AGCAGTGGCT CCGCCAGTGA CCGCACGTCC TCCAACCCCT 901 TCAGCGGCCG CTCGGCAGGC AGCCACCCCG GTACCCGAAC TGACGGCTGC CCAACTTTCT 961 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1021 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1081 GTGGAAAGGA CGAGCAAAGA CACGTAGACG ATAAGCCACG CGTTAAGTCg acaatcaacc 1141 tctggattac aaaatttgtg aaagatt