Construct: ORF TRCN0000473716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005639.1_s317c1
- Derived from:
- ccsbBroadEn_11381
- DNA Barcode:
- ACCTCAACCCCCCAACTTCCCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCAMP1 (9522)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9522 | SCAMP1 | secretory carrier membrane ... | XM_011543727.3 | 51.6% | 51.6% | 472_912del |
| 2 | human | 9522 | SCAMP1 | secretory carrier membrane ... | NM_004866.6 | 46.4% | 46.4% | 472_1014del |
| 3 | human | 9522 | SCAMP1 | secretory carrier membrane ... | NM_001290229.2 | 38.7% | 38.7% | 57_58ins78;394_936del |
| 4 | human | 9522 | SCAMP1 | secretory carrier membrane ... | NR_110885.2 | 7.8% | 1_55del;527_5983del | |
| 5 | mouse | 107767 | Scamp1 | secretory carrier membrane ... | NM_029153.1 | 40.5% | 45.8% | (many diffs) |
| 6 | mouse | 107767 | Scamp1 | secretory carrier membrane ... | NM_001346609.1 | 27.3% | 30.7% | (many diffs) |
| 7 | mouse | 107767 | Scamp1 | secretory carrier membrane ... | XM_006517515.2 | 27.3% | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggatttcgac agtaacccgt ttgccgaccc ggatctcaac aatcccttca 121 aggatccatc agttacacaa gtgaCAAGAA ATGTTCCACC AGGACTTGAT GAATATAATC 181 CATTCTCGGA TTCTAGAACA CCTCCACCAG GCGGTGTGAA GATGCCTAAT GTACCCAATA 241 CACAACCAGC AATAATGAAA CCAACAGAGG AACATCCAGC TTATACACAG ATTGCAAAGG 301 AACATGCATT GGCCCAAGCT GAACTTCTTA AGCGCCAGGA AGAACTAGAA AGAAAAGCCG 361 CAGAATTAGA TCGTCGGGAA CGAGAAATGC AAAACCTCAG TCAACATGGT AGAAAAAATA 421 ATTGGCCACC TCTTCCTAGC AATTTTCCTG TCGGACCTTG TTTCTATCAG GATTTTTCTG 481 TAGACATTCC TGTAGAATTC CAAAAGACAG TAAAGCTTAT GTACTACTTG TGGATGTGCC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GAACCTCAAC CCCCCAACTT CCCTGCACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt