Construct: ORF TRCN0000473716

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005639.1_s317c1
Derived from:
ccsbBroadEn_11381
DNA Barcode:
ACCTCAACCCCCCAACTTCCCTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCAMP1 (9522)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473716
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9522 SCAMP1 secretory carrier membrane ... XM_011543727.3 51.6% 51.6% 472_912del
2 human 9522 SCAMP1 secretory carrier membrane ... NM_004866.6 46.4% 46.4% 472_1014del
3 human 9522 SCAMP1 secretory carrier membrane ... NM_001290229.2 38.7% 38.7% 57_58ins78;394_936del
4 human 9522 SCAMP1 secretory carrier membrane ... NR_110885.2 7.8% 1_55del;527_5983del
5 mouse 107767 Scamp1 secretory carrier membrane ... NM_029153.1 40.5% 45.8% (many diffs)
6 mouse 107767 Scamp1 secretory carrier membrane ... NM_001346609.1 27.3% 30.7% (many diffs)
7 mouse 107767 Scamp1 secretory carrier membrane ... XM_006517515.2 27.3% 30.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
537
ORF length:
471
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ggatttcgac agtaacccgt ttgccgaccc ggatctcaac aatcccttca
121aggatccatc agttacacaa gtgaCAAGAA ATGTTCCACC AGGACTTGAT GAATATAATC
181CATTCTCGGA TTCTAGAACA CCTCCACCAG GCGGTGTGAA GATGCCTAAT GTACCCAATA
241CACAACCAGC AATAATGAAA CCAACAGAGG AACATCCAGC TTATACACAG ATTGCAAAGG
301AACATGCATT GGCCCAAGCT GAACTTCTTA AGCGCCAGGA AGAACTAGAA AGAAAAGCCG
361CAGAATTAGA TCGTCGGGAA CGAGAAATGC AAAACCTCAG TCAACATGGT AGAAAAAATA
421ATTGGCCACC TCTTCCTAGC AATTTTCCTG TCGGACCTTG TTTCTATCAG GATTTTTCTG
481TAGACATTCC TGTAGAATTC CAAAAGACAG TAAAGCTTAT GTACTACTTG TGGATGTGCC
541CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
601TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
661ATATATCTTG TGGAAAGGAC GAACCTCAAC CCCCCAACTT CCCTGCACGC GTTAAGTCga
721caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)