Construct: ORF TRCN0000473748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008662.1_s317c1
- Derived from:
- ccsbBroadEn_04243
- DNA Barcode:
- GCAGTAAATTGTCACTGTTAACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLA2G12A (81579)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81579 | PLA2G12A | phospholipase A2 group XIIA | NM_030821.5 | 100% | 100% | |
| 2 | mouse | 66350 | Pla2g12a | phospholipase A2, group XIIA | NM_023196.4 | 81.8% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgctctcg cgccccgcgc tcaccctcct gctcctcctc atggccgctg 121 ttgtcaggtg ccaggagcag gcccagacca ccgactggag agccaccctg aagaccatcc 181 ggaacggcgt tcataagata gacacgtacc tgaacgccgc cttggacctc ctgggaggcg 241 aggacggtcT CTGCCAGTAT AAATGCAGTG ACGGATCTAA GCCTTTCCCA CGTTATGGTT 301 ATAAACCCTC CCCACCGAAT GGATGTGGCT CTCCACTGTT TGGTGTTCAT CTTAACATTG 361 GTATCCCTTC CCTGACAAAG TGTTGCAACC AACACGACAG GTGCTATGAG ACCTGTGGCA 421 AAAGCAAGAA TGACTGTGAT GAAGAATTCC AGTATTGCCT CTCCAAGATC TGCCGAGATG 481 TACAGAAAAC ACTAGGACTA ACTCAGCATG TTCAGGCATG TGAAACAACA GTGGAGCTCT 541 TGTTTGACAG TGTTATACAT TTAGGTTGTA AACCATATCT GGACAGCCAA CGAGCCGCAT 601 GCAGGTGTCA TTATGAAGAA AAAACTGATC TTTACCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 781 AGTAAATTGT CACTGTTAAC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt