Construct: ORF TRCN0000473753
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012950.1_s317c1
- Derived from:
- ccsbBroadEn_08308
- DNA Barcode:
- ATGCAATAGCTATTGGGGTATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRMO (51531)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473753
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51531 | TRMO | tRNA methyltransferase O | NM_001371657.1 | 99.7% | 99.7% | 19T>C;57T>G;276T>C |
| 2 | human | 51531 | TRMO | tRNA methyltransferase O | NM_016481.5 | 99.7% | 99.7% | 19T>C;57T>G;276T>C |
| 3 | human | 51531 | TRMO | tRNA methyltransferase O | NM_001371658.1 | 86.3% | 81.6% | (many diffs) |
| 4 | human | 51531 | TRMO | tRNA methyltransferase O | XM_011518776.3 | 83.3% | 78.7% | (many diffs) |
| 5 | human | 51531 | TRMO | tRNA methyltransferase O | NM_001371659.1 | 83.1% | 83.2% | 0_1ins222;54T>C |
| 6 | human | 51531 | TRMO | tRNA methyltransferase O | XM_011518777.1 | 72.6% | 68.2% | (many diffs) |
| 7 | human | 51531 | TRMO | tRNA methyltransferase O | NM_001330725.2 | 66.8% | 66.8% | 0_1ins438 |
| 8 | human | 51531 | TRMO | tRNA methyltransferase O | NM_001371660.1 | 66.8% | 66.8% | 0_1ins438 |
| 9 | human | 51531 | TRMO | tRNA methyltransferase O | NM_001371661.1 | 66.8% | 66.8% | 0_1ins438 |
| 10 | human | 51531 | TRMO | tRNA methyltransferase O | XM_024447569.1 | 66.8% | 66.8% | 0_1ins438 |
| 11 | human | 51531 | TRMO | tRNA methyltransferase O | XR_929808.3 | 39.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1389
- ORF length:
- 1323
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg cggcttggag gagccggggc ctcggcctac agcgaccccg tgcggctgcg 121 tgaagccggc tctggagaca gggaatcttt taactgagcc agtcggctac ttggaatctt 181 gtttctcggc caagaatggt actccaagac agccatccat ttgtagctat tctcgagcct 241 gtttgaggat tagaaagagg atctttaata atcctgaaca ttccttgatg ggcctagaac 301 agttttctca tgtttggatt ttgtttgttt ttcacaaaaa cggtcatttg agctgtaagg 361 caaaagtgca gcctcctagg ctgaatggtg caaagactgg agttttttcc acaaggagcc 421 ctcatcgtcc caatgcaata ggactgaccc tggccaagct ggaaaaggta gaaggtggag 481 ctatatacct ttctggaatt gacatgatac atggcacacc cgtactagac atcaagccct 541 acatagctga gtatgactca ccgcaaaatg tgatggagcc tttagcagac tttaatttac 601 agaataacca acatacacca aacactgtgt cccagtctga cagcaagact gacagctgtg 661 accagcgaca gctctcaggg tgtgatgagc cacaacccca ccatagcact aagaggaaac 721 ctaaatgtcc tgaagacaga acttcagaag aaaactacct gacacacagt gacacagcca 781 gaattcagca agcatttcct atgcacaggg agatagcagt ggattttggt ttggaatcaa 841 gacgtgaTCA GAGTTCCAGC GTGGCAGAAG AACAAATTGG CCCATATTGC CCAGAGAAGA 901 GCTTTTCAGA GAAAGGTACA GACAAGAAGC TAGAAAGAGT GGAAGGAGCA GCAGTCTTGC 961 AAGGAAGCAG GGCAGAGACA CAGCCCATGG CCCCTCACTG CCCTGCTGGA AGGGCTGATG 1021 GAGCTCCCCG CAGCGTGGTT CCTGCCTGGG TGACAGAGGC TCCTGTGGCC ACTTTAGAAG 1081 TGCGGTTTAC TCCTCATGCC GAGATGGACC TTGGGCAGCT CAGTTCACAA GATGTTGGTC 1141 AGGCGTCATT TAAATATTTT CAGTCAGCAG AGGAAGCAAA GCGTGCCATT GAGGCTGTGC 1201 TGTCAGCGGA TCCTCGGTCT GTGTACCGCC GGAAGCTTTG CCAGGACCGC CTTTTCTACT 1261 TTACTGTAGA CATAGCGCAT GTCACTTGCT GGTTTGGTGA TGGCTTTGCA GAGGTGCTGA 1321 GGATCAAGCC GGCTTCTGAG CCTGTTCATA TGACTGGCCC TGTGGGGTCC TTGGTGTCTC 1381 TAGGGTCTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1441 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1501 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATGCAA TAGCTATTGG GGTATACCAC 1561 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt