Construct: ORF TRCN0000473758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001817.1_s317c1
- Derived from:
- ccsbBroadEn_12604
- DNA Barcode:
- ACTGGGCTGGGAAATTGCTTCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN7 (79730)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473758
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79730 | NSUN7 | NOP2/Sun RNA methyltransfer... | NM_001330648.3 | 99.8% | 99.7% | 906C>T;922T>G |
| 2 | human | 79730 | NSUN7 | NOP2/Sun RNA methyltransfer... | XM_017008613.1 | 97.9% | 98.1% | (many diffs) |
| 3 | human | 79730 | NSUN7 | NOP2/Sun RNA methyltransfer... | XM_017008615.1 | 81.2% | 75.8% | (many diffs) |
| 4 | human | 79730 | NSUN7 | NOP2/Sun RNA methyltransfer... | NM_024677.6 | 66% | 65.1% | (many diffs) |
| 5 | human | 79730 | NSUN7 | NOP2/Sun RNA methyltransfer... | XM_017008611.2 | 59.3% | 58.4% | (many diffs) |
| 6 | human | 79730 | NSUN7 | NOP2/Sun RNA methyltransfer... | XM_017008612.2 | 54.6% | 53.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gaattccacg ggcgaactgg agttttcgaa cgaagaagat cccgagatca 121 tctcccaact cacttccctg cctctgtccg gtgggaaaag ctcagctggt gtgcccgaaa 181 aaacgggcta tccggactcc gtttatgtca tggcagccaa catttttcag ggtattcgaa 241 tcgaaaagtc ggcacagaaa gtcttaatca agtatgggaa tgaacccctg cggtccttgt 301 ccgagtctga ggatcagtcc tttcagcgtt tgtcttatga gctggctttc agtgccctga 361 aatatcaaga tattttggaa actatattga tagacagctg tatcttccca agtaccacaa 421 taccagatca tttgagcagt cttattattg tgatgctata tgatttccaa gatagaaaat 481 ttcaaactcg tgtcctttct gataatgaag agcccatatc agaagttcaa gaagtagaga 541 accttcttaa cagttttaag ataaaattgg ctgcagcatt ggcaagatgt cgaatcaagc 601 atgatgccct ttcaatttac cacatccttc cagaaacagt taggaaacag gaactaaggg 661 cctccacttt accactttat gcttggataa atacttgtaa aatcagccct gaagaagttt 721 ataataattt gaagagaaga ggctataata aagtcaaatc tgtattgcat attgatgata 781 aagtctttgc tgtggatcaa cattgctatg atgtcttaat ttttccatct catcttaaaa 841 atgatcttat aaatatagat cttttcaaag attacaaact tatatttcag gacaaatctc 901 gaagtcttgc tgtccattct gtaaaggctt tattaaatat ggatgatgat GTCTTAATGG 961 TCAATACAGG TTCATGGTAC ACAGTTGCCC ACATGTCAAT TTTAACAAAT AATAATACCT 1021 CAAAAGTATT TGTGTGTGGA GTACAATCAC AAGCTAAGGA TCCTGACTTG AAGACCCTTT 1081 TCACAAAAAT AGGATGTAAA AATATTGAAA TACTTCATGA GAAATTTATT AACATTGAAT 1141 CAAAGGATCA CAGGTTACAG AAAGTTAAAG TGATTTTGCT GCTACCTCGT TGTTCAGGAC 1201 TGGGTGTTAG TAATCCAGTA GAATTTATTT TAAATGAACA TGAAGATACA GAATTCCTTA 1261 AAGATCACTC TCAAGGAGGC ATCTCAGTGG ACAAACTTCA CGTTCTTGCT CAACAGCAGT 1321 ATGAACAGCT AACACATGCA ATGAAATTTA CTAAAGCTCA AGCAGTTGTT TACTGCACAT 1381 GTTCAGTTTT TCCAGAAGAA AATGAAGCTG TTGTTAAGAA AGCACTGGAA TTTCAAGACC 1441 TTGGGAATAA AGGACAACCT TACAGCGGGA CCCTTCTGAG ACAGTGTCTG TGCCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTT ATTTCGATTT CTTGGCTTTA TATATCTTGT 1621 GGAAAGGACG AACTGGGCTG GGAAATTGCT TCGAAACGCG TTAAGTCgac aatcaacctc 1681 tggattacaa aatttgtgaa agatt