Construct: ORF TRCN0000473763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001950.1_s317c1
- Derived from:
- ccsbBroadEn_06054
- DNA Barcode:
- GCGATCGGTAATAGCTCCCTTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSN3 (1448)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473763
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1448 | CSN3 | casein kappa | NM_005212.2 | 99.6% | 99.4% | 6G>A;224A>G |
| 2 | human | 1448 | CSN3 | casein kappa | XM_011531620.2 | 99.6% | 99.4% | 6G>A;224A>G |
| 3 | human | 1448 | CSN3 | casein kappa | XM_017007761.1 | 99.6% | 99.4% | 6G>A;224A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aagttttctt ctagttgtca atgccctggc attaaccctg ccttttttgg 121 ctgtggaggt tcaaaaCCAG AAACAACCAG CATGCCATGA GAATGATGAA AGACCATTCT 181 ATCAGAAAAC AGCTCCATAT GTCCCAATGT ATTATGTGCC AAATAGCTAT CCTTATTATG 241 GAACCAATTT GTACCAACGT AGACCAGCTA TAGCAATTAA TAATCCATGT GTGCCTCGCA 301 CATATTATGC AAACCCAGCT GTAGTTAGGC CACATGCCCA AATTCCTCAG CGGCAATACC 361 TGCCAAATAG CCACCCACCC ACTGTGGTAC GTCGCCCAAA CCTGCATCCA TCATTTATTG 421 CCATCCCCCC AAAGAAAATT CAGGATAAAA TAATCATCCC TACCATCAAT ACCATTGCTA 481 CTGTTGAACC TACACCAGCT CCTGCCACTG AACCAACGGT GGACAGTGTA GTCACTCCAG 541 AAGCTTTTTC AGAGTCCATC ATCACGAGCA CCCCTGAGAC AACCACAGTT GCAGTTACTC 601 CACCTACGGC ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCG ATCGGTAATA GCTCCCTTCG 781 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t