Construct: ORF TRCN0000473764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013162.1_s317c1
- Derived from:
- ccsbBroadEn_09106
- DNA Barcode:
- TCCTAACCGCTTGGCTGCGCGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRISPLD1 (83690)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83690 | CRISPLD1 | cysteine rich secretory pro... | NM_031461.6 | 99.9% | 100% | 15G>T |
| 2 | human | 83690 | CRISPLD1 | cysteine rich secretory pro... | NM_001286777.2 | 62.8% | 59% | 0_1ins442;68_69ins116 |
| 3 | human | 83690 | CRISPLD1 | cysteine rich secretory pro... | NM_001286778.2 | 62.4% | 62.4% | 0_1ins564 |
| 4 | mouse | 83691 | Crispld1 | cysteine-rich secretory pro... | NM_001356551.1 | 88.4% | 94.6% | (many diffs) |
| 5 | mouse | 83691 | Crispld1 | cysteine-rich secretory pro... | NM_031402.2 | 88.4% | 94.6% | (many diffs) |
| 6 | mouse | 83691 | Crispld1 | cysteine-rich secretory pro... | XM_006495592.3 | 88.4% | 94.6% | (many diffs) |
| 7 | mouse | 83691 | Crispld1 | cysteine-rich secretory pro... | XM_011238409.3 | 73.7% | 79% | (many diffs) |
| 8 | mouse | 83691 | Crispld1 | cysteine-rich secretory pro... | XM_006495594.4 | 70.4% | 76% | (many diffs) |
| 9 | mouse | 83691 | Crispld1 | cysteine-rich secretory pro... | XM_011238410.2 | 55.6% | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtgtaccgct cgggagtggc tcagagtaac cacagtgctg ttcatggcta 121 gagcaattcc agccatggtg gttcccaatg ccactttatt ggagaaactt ttggaaaaat 181 acatggatga ggatggtgag tggtggatag ccaaacaacg agggaaaagg gccatcacag 241 acaatgacat gcagagtatt ttggaccttc ataataaatt acgaagtcag gtgtatccaa 301 cagcctctaa tatggagtat atgacatggg atgtagagct ggaaagatct gcagaatcct 361 gggctgaaag ttgcttgtgg gaacatggac ctgcaagctt gcttccatca attggacaga 421 atttgggagc acactgggga agatataggc ccccgacgtt tcatgtacaa tcgtggtatg 481 atgaagtgaa agactttagc tacccatatg aacatgaatg caacccatat tgtccattca 541 ggtgttctgg ccctgtatgt acacattata cacaggtcgt gtgggcaact agtaacagaa 601 tcggttgtgc cattaatttg tgtcataaca tgaacatctg ggggcagata tggcccaaag 661 ctgtctacct ggtgtgcaat tactccccaa agggaaactg gtggggccat gccccttaca 721 aacatgggcg gccctgttct gcttgcccac ctagttttgg agggggctgt agagaaaatc 781 tgtgctacaa agaagggtca gacaggtatt atccccctcg agaagaggaa acaaatgaaa 841 tagaacgaca gcagtcacaa gtccatgaca cccatgtccg gacaagatca gatgatagta 901 gcagaaatga agtcataagc gcacagcaaa tgtcccaaat tgtttcttgt gaagtaagat 961 taagagatca gtgcaaagga acaacctgca ataggtacga atgtcCTGCT GGCTGTTTGG 1021 ATAGTAAAGC TAAAGTTATT GGCAGTGTAC ATTATGAAAT GCAATCCAGC ATCTGTAGAG 1081 CTGCAATTCA TTATGGTATA ATAGACAATG ATGGTGGCTG GGTAGATATC ACTAGACAAG 1141 GAAGAAAGCA TTATTTCATC AAGTCCAATA GAAATGGTAT TCAAACAATT GGCAAATATC 1201 AGTCTGCTAA TTCCTTCACA GTCTCTAAAG TAACAGTTCA GGCTGTGACT TGTGAAACAA 1261 CTGTGGAACA GCTCTGTCCA TTTCATAAGC CTGCTTCACA TTGCCCAAGA GTATACTGTC 1321 CTCGTAACTG TATGCAAGCA AATCCACATT ATGCTCGTGT AATTGGAACT CGAGTTTATT 1381 CTGATCTGTC CAGTATCTGC AGAGCAGCAG TACATGCTGG AGTGGTTCGA AATCACGGTG 1441 GTTATGTTGA TGTAATGCCT GTGGACAAAA GAAAGACCTA CATTGCTTCT TTTCAGAATG 1501 GAATCTTCTC AGAAAGTTTA CAGAATCCTC CAGGAGGAAA GGCATTCAGA GTGTTTGCTG 1561 TTGTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCTAACCG CTTGGCTGCG CGCGGACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt