Construct: ORF TRCN0000473775
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008586.1_s317c1
- Derived from:
- ccsbBroadEn_02526
- DNA Barcode:
- TGAAGTCGCCGACTGTATTATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POP4 (10775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473775
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10775 | POP4 | POP4 homolog, ribonuclease ... | NM_006627.3 | 100% | 100% | |
| 2 | human | 10775 | POP4 | POP4 homolog, ribonuclease ... | NR_027368.2 | 17% | 1_36del;94_95ins224;473_2332del | |
| 3 | mouse | 66161 | Pop4 | processing of precursor 4, ... | XM_006540287.3 | 69.7% | 69.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 726
- ORF length:
- 660
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gagtgtgatc taccatgcat tgtctcagaa agaggcgaat gactccgatg 121 tccagccttc aggagcacag cgggccgagg ccttcgtgag ggccttcctg aagcgcagca 181 cgccccgcat gagcccgcag gcccgcgagg accagctgca gcgcaaggcg gtggtcctgg 241 agtacttcac ccgccacaag cgcaaggaga agaagaagaa agccaaaggc ctctctgcca 301 ggcaaaggag ggagctgcgg ctctttgaca ttaaaccaga gcagcagaga tacagCCTTT 361 TCCTCCCTCT CCATGAACTC TGGAAACAGT ACATCAGGGA CCTGTGCAGT GGGCTCAAGC 421 CAGACACGCA GCCACAGATG ATTCAGGCCA AGCTCTTAAA GGCAGATCTT CACGGGGCTA 481 TTATTTCAGT GACAAAATCC AAATGCCCCT CTTATGTGGG TATTACAGGA ATCCTTCTAC 541 AGGAAACAAA GCACATTTTC AAAATTATCA CCAAAGAAGA CCGCCTGAAA GTTATCCCCA 601 AGCTAAACTG CGTGTTCACT GTGGAAACCG ATGGCTTTAT TTCCTACATT TACGGGAGCA 661 AATTCCAGCT TCGGTCAAGT GAACGGTCTG CGAAGAAGTT CAAAGCGAAG GGAACGATTG 721 ACCTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGAAGTCGC CGACTGTATT ATACCACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt