Construct: ORF TRCN0000473781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009876.1_s317c1
- Derived from:
- ccsbBroadEn_01515
- DNA Barcode:
- CCTCCAGTCAGAGTCATCAAGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHD (6392)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_003002.4 | 100% | 100% | |
| 2 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_001276506.2 | 78.2% | 69.5% | (many diffs) |
| 3 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_001276504.2 | 75.4% | 75.4% | 52_53ins117 |
| 4 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_001276503.2 | 53.4% | 39.6% | 169_170ins145;255_256ins77 |
| 5 | human | 6392 | SDHD | succinate dehydrogenase com... | NR_077060.2 | 34.2% | 1_35del;350_403del;567_1393del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 567
- ORF length:
- 477
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGGTTCT CTGGAGGCTG AGTGCCGTTT 121 GCGGTGCCCT AGGAGGCCGA GCTCTGTTGC TTCGAACTCC AGTGGTCAGA CCTGCTCATA 181 TCTCAGCATT TCTTCAGGAC CGACCTATCC CAGAATGGTG TGGAGTGCAG CACATACACT 241 TGTCACCGAG CCACCATTCT GGCTCCAAGG CTGCATCTCT CCACTGGACT AGCGAGAGGG 301 TTGTCAGTGT TTTGCTCCTG GGTCTGCTTC CGGCTGCTTA TTTGAATCCT TGCTCTGCGA 361 TGGACTATTC CCTGGCTGCA GCCCTCACTC TTCATGGTCA CTGGGGCCTT GGACAAGTTG 421 TTACTGACTA TGTTCATGGG GATGCCTTGC AGAAAGCTGC CAAGGCAGGG CTTTTGGCAC 481 TTTCAGCTTT AACCTTTGCT GGGCTTTGCT ATTTCAACTA TCACGATGTG GGCATCTGCA 541 AAGCTGTTGC CATGCTGTGG AAGCTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCTCCAGT 721 CAGAGTCATC AAGCAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt