Construct: ORF TRCN0000473784
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012642.1_s317c1
- Derived from:
- ccsbBroadEn_13721
- DNA Barcode:
- TGACACCATACCTCCCCAATACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC643406 (643406)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473784
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 643406 | LOC643406 | uncharacterized LOC643406 | NR_029405.1 | 7.6% | (many diffs) | |
| 2 | human | 149837 | LINC00654 | long intergenic non-protein... | NR_015406.2 | 4.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 268
- ORF end:
- 562
- ORF length:
- 294
- Sequence:
-
1 TCGATTAGTT CTCGAGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG 61 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA 121 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC 181 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGCTAG CATCGATTGA 241 TCAACAAGTT TGTACAAAAA AGTTGGCATG ACCGCCTTTG CACTGTCCAT GCTCTCAGCC 301 CACCACCTCC TCCCCCTGCC ATTGCAGTGG CTAACACTGG AGACGAAGAC CAAGACACCA 361 CCGCCTTTCT CCAGTACCTC TCAAATCAGC ACAGGCAAGG ACAAAGGCCT CAATCCACAA 421 TTGCTGAAGA TGGACCCTGG CCACATGGGA TGGTCAGACA CGCCTGCCCA GCTATCTGCA 481 GGCGAAGAGG CTCAGAAGAG GTTTAGGGGC CTGAAGGACA TCTTGCTTCC ATGTCCATAT 541 GAGCAGGCTA TTTCTGCTCC ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 601 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 661 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGA CACCATACCT 721 CCCCAATACC AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 781 t