Construct: ORF TRCN0000473785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014842.1_s317c1
- Derived from:
- ccsbBroadEn_05837
- DNA Barcode:
- AAGGACACTACGCGCTAAATACTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- APOC1 (341)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473785
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 341 | APOC1 | apolipoprotein C1 | NM_001321065.2 | 99.5% | 92.7% | 232A>T |
| 2 | human | 341 | APOC1 | apolipoprotein C1 | NM_001321066.2 | 99.5% | 92.7% | 232A>T |
| 3 | human | 341 | APOC1 | apolipoprotein C1 | NM_001645.5 | 99.5% | 92.7% | 232A>T |
| 4 | human | 342 | APOC1P1 | apolipoprotein C1 pseudogene 1 | NR_028414.1 | 35.1% | (many diffs) | |
| 5 | human | 342 | APOC1P1 | apolipoprotein C1 pseudogene 1 | NR_028413.1 | 29.4% | (many diffs) | |
| 6 | human | 342 | APOC1P1 | apolipoprotein C1 pseudogene 1 | NR_028412.1 | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 237
- ORF end:
- 468
- ORF length:
- 231
- Sequence:
-
1 GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA 61 GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA 121 GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT 181 TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGA 241 GGCTCTTCCT GTCGCTCCCG GTCCTGGTGG TGGTTCTGTC GATCGTCTTG GAAGGCCCAG 301 CCCCAGCCCA GGGGACCCCA GACGTCTCCA GTGCCTTGGA TAAGCTGAAG GAGTTTGGAA 361 ACACACTGGA GGACAAGGCT CGGGAACTCA TCAGCCGCAT CAAACAGAGT GAACTTTCTG 421 CCAAGATGCG GGAGTGGTTT TCAGAGACAT TTCAGAAAGT GAAGGAGTAA CTCAAGATTG 481 ACTCATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAAGGACACT ACGCGCTAAA TACTAACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt